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Literature summary for 2.4.1.41 extracted from
- Purification and characterization of UDP-N-acetylgalactosamine: polypeptide N-acetylgalactosaminyltransferase from bovine colostrum and murine lymphoma BW5147 cells (1986), J. Biol. Chem., 261, 5249-5255.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 0.5 mM, complete inhibition | Bos taurus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Mus musculus | |
| additional information | - |
additional information | Km: 2.5 mg/ml: deglycosylated bovine submaxillary mucin | Bos taurus | |
| 0.008 | - |
UDP-GalNAc | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane-bound | Mus musculus | 16020 | - |
| membrane | membrane-bound | Bos taurus | 16020 | - |
| soluble | may be a proteolytic cleavage product from a membrane-bound form | Bos taurus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | partially restores activity after EDTA treatment | Bos taurus | |
| Co2+ | almost as effective as Mn2+, restores activity after EDTA treatment | Bos taurus | |
| Mn2+ | requirement | Mus musculus | |
| Mn2+ | requirement | Bos taurus | |
| Mn2+ | maximal activity at 2.5 mM, restores activity after EDTA treatment | Bos taurus | |
| Mn2+ | most effective cation | Bos taurus | |
| Ni2+ | partially restores activity after EDTA treatment | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69000 | - |
gel filtration | Bos taurus |
| 70000 | - |
1 * 70000, SDS-PAGE | Mus musculus |
| 70000 | - |
1 * 70000, SDS-PAGE | Bos taurus |
| 71500 | - |
gel filtration | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-galactosamine + polypeptide | Mus musculus | key role in O-linked glycosylation, catalyzes first step in the assembly | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
| UDP-N-acetyl-D-galactosamine + polypeptide | Bos taurus | key role in O-linked glycosylation, catalyzes first step in the assembly | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
colostrum | - |
| Mus musculus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 2 N-linked oligosaccharides, on most enzyme molecules both oligosaccharides are of the complex type, but some molecules contain one complex type and one high mannose type | Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2500fold | Mus musculus |
| 517000fold | Bos taurus |
| partial | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| BW-5147 cell | lymphoma cells | Mus musculus | - |
| colostrum | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.15 | - |
- |
Mus musculus |
| 1.86 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| enzyme loses activity upon storage | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | - |
Mus musculus | ? | - |
? | |
| additional information | not: UDP-Glc | Bos taurus | ? | - |
? | |
| additional information | not: UDP-GlcNAc | Bos taurus | ? | - |
? | |
| additional information | not: UDP-Gal | Bos taurus | ? | - |
? | |
| UDP-N-acetyl-D-galactosamine + apomucin | deglycosylated bovine submaxillary mucin | Bos taurus | UDP + N-acetyl-D-galactosaminyl-apomucin | - |
? | |
| UDP-N-acetyl-D-galactosamine + polypeptide | acceptor: deglycosylated bovine submaxillary mucin | Bos taurus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
| UDP-N-acetyl-D-galactosamine + polypeptide | only UDP-GalNAc serves as sugar donor | Mus musculus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
| UDP-N-acetyl-D-galactosamine + polypeptide | only UDP-GalNAc serves as sugar donor | Bos taurus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
| UDP-N-acetyl-D-galactosamine + polypeptide | key role in O-linked glycosylation, catalyzes first step in the assembly | Mus musculus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
| UDP-N-acetyl-D-galactosamine + polypeptide | key role in O-linked glycosylation, catalyzes first step in the assembly | Bos taurus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 70000, SDS-PAGE | Mus musculus |
| monomer | 1 * 70000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
| 37 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 8.6 | - |
Bos taurus |
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