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Literature summary for 2.4.1.40 extracted from

  • Yamamoto, F.; Yamamoto, M.; Blancher, A.
    Generation of histo-blood group B transferase by replacing the N-acetyl-D-galactosamine recognition domain of human A transferase with the galactose-recognition domain of evolutionarily related murine alpha1,3-galactosyltransferase (2009), Transfusion, 50, 622-630.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information generation of a chimeric histo-blood group B transferase by replacing the N-acetyl-D-galactosamine recognition domain of human A transferase with the galactose-recognition domain of evolutionarily related murine alpha1,3-galactosyltransferase, the chimeric mutant shows a functional conversion from A to B transferase activity, overview. The AT-MluI-GT construct, which expresses the chimera between the long N-terminal sequence up to the MluI site of human A transferase, and the short C-terminal sequence up to the MluI site of murine a1-3Gal transferase, produces the antigens that react with both the anti-A and the Griffonia simplicifolia Lectin I, GSL-IA4, lectin Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1,2)-D-galactose Homo sapiens substrate is the histo-blood H group antigen UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactose product is the histo-blood A group antigen ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
A transferase
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1,2)-D-galactose substrate is the histo-blood H group antigen Homo sapiens UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactose product is the histo-blood A group antigen ?
UDP-N-acetyl-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1,2)-D-galactose substrate is the histo-blood H group antigen. Arg176 of the enzyme is not important in determining the sugar specificity of enzyme, while Gly235 is influential and Leu266 and Gly268 are crucial in the determination of GalNAc/galactose specificity Homo sapiens UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactose product is the histo-blood A group antigen ?

Synonyms

Synonyms Comment Organism
A transferase
-
Homo sapiens
ABO
-
Homo sapiens
More the A/B transferases, ABO, belong to the GT6 enzyme family Homo sapiens