Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H195A | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
H195Q | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
H195R | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
R226A | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
R226K | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
R270A | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
R270K | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
Y194A | inactive | Homo sapiens |
Y194F | inactive | Homo sapiens |
Y196A | inactive | Homo sapiens |
Y196F | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
Y199A | inactive | Homo sapiens |
Y199F | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-methylumbelliferyl 4-deoxy-4-fluoro-beta-D-xylopyranoside | - |
Homo sapiens | |
4-methylumbelliferyl 4-deoxy-beta-D-xylopyranoside | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
UDP-alpha-D-galactose | recombinant wild type enzyme, at pH 7.0 and 37°C | Homo sapiens | |
0.27 | - |
UDP-alpha-D-galactose | mutant enzyme R270A, at pH 7.0 and 37°C | Homo sapiens | |
0.29 | - |
UDP-alpha-D-galactose | mutant enzyme R226K, at pH 7.0 and 37°C | Homo sapiens | |
0.32 | - |
UDP-alpha-D-galactose | mutant enzyme H195R, at pH 7.0 and 37°C | Homo sapiens | |
0.32 | - |
UDP-alpha-D-galactose | mutant enzyme Y199F, at pH 7.0 and 37°C | Homo sapiens | |
0.33 | - |
UDP-alpha-D-galactose | mutant enzyme H195Q, at pH 7.0 and 37°C | Homo sapiens | |
0.34 | - |
UDP-alpha-D-galactose | mutant enzyme R226A, at pH 7.0 and 37°C | Homo sapiens | |
0.34 | - |
UDP-alpha-D-galactose | mutant enzyme Y196F, at pH 7.0 and 37°C | Homo sapiens | |
0.35 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme H195R, at pH 7.0 and 37°C | Homo sapiens | |
0.35 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | recombinant wild type enzyme, at pH 7.0 and 37°C | Homo sapiens | |
0.37 | - |
UDP-alpha-D-galactose | mutant enzyme R270K, at pH 7.0 and 37°C | Homo sapiens | |
0.4 | - |
UDP-alpha-D-galactose | mutant enzyme H195A, at pH 7.0 and 37°C | Homo sapiens | |
0.44 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme R226A, at pH 7.0 and 37°C | Homo sapiens | |
0.46 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme R226K, at pH 7.0 and 37°C | Homo sapiens | |
0.54 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme R270K, at pH 7.0 and 37°C | Homo sapiens | |
0.55 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme H195Q, at pH 7.0 and 37°C | Homo sapiens | |
0.59 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme Y199F, at pH 7.0 and 37°C | Homo sapiens | |
0.6 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme R270A, at pH 7.0 and 37°C | Homo sapiens | |
0.64 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme H195A, at pH 7.0 and 37°C | Homo sapiens | |
1.06 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme Y196F, at pH 7.0 and 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UBV7 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-galactose + 4-methylumbelliferyl-beta-D-xylopyranoside | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta1,4-galactosyltransferase 7 | - |
Homo sapiens |
beta4GalT7 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
UDP-alpha-D-galactose | mutant enzyme Y196F, at pH 7.0 and 37°C | Homo sapiens | |
0.5 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme Y196F, at pH 7.0 and 37°C | Homo sapiens | |
0.77 | - |
UDP-alpha-D-galactose | mutant enzyme R270A, at pH 7.0 and 37°C | Homo sapiens | |
0.77 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme R270A, at pH 7.0 and 37°C | Homo sapiens | |
0.82 | - |
UDP-alpha-D-galactose | mutant enzyme R270K, at pH 7.0 and 37°C | Homo sapiens | |
0.82 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme R270K, at pH 7.0 and 37°C | Homo sapiens | |
0.89 | - |
UDP-alpha-D-galactose | mutant enzyme R226A, at pH 7.0 and 37°C | Homo sapiens | |
0.89 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme R226A, at pH 7.0 and 37°C | Homo sapiens | |
1.2 | - |
UDP-alpha-D-galactose | mutant enzyme Y199F, at pH 7.0 and 37°C | Homo sapiens | |
1.2 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme Y199F, at pH 7.0 and 37°C | Homo sapiens | |
1.35 | - |
UDP-alpha-D-galactose | mutant enzyme R226K, at pH 7.0 and 37°C | Homo sapiens | |
1.35 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme R226K, at pH 7.0 and 37°C | Homo sapiens | |
1.49 | - |
UDP-alpha-D-galactose | mutant enzyme H195R, at pH 7.0 and 37°C | Homo sapiens | |
1.49 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme H195R, at pH 7.0 and 37°C | Homo sapiens | |
1.51 | - |
UDP-alpha-D-galactose | recombinant wild type enzyme, at pH 7.0 and 37°C | Homo sapiens | |
1.51 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | recombinant wild type enzyme, at pH 7.0 and 37°C | Homo sapiens | |
1.62 | - |
UDP-alpha-D-galactose | mutant enzyme H195Q, at pH 7.0 and 37°C | Homo sapiens | |
1.62 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme H195Q, at pH 7.0 and 37°C | Homo sapiens | |
1.93 | - |
UDP-alpha-D-galactose | mutant enzyme H195A, at pH 7.0 and 37°C | Homo sapiens | |
1.93 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme H195A, at pH 7.0 and 37°C | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
4-methylumbelliferyl 4-deoxy-4-fluoro-beta-D-xylopyranoside | recombinant wild type enzyme, at pH 7.0 and 37°C | Homo sapiens | |
1.28 | - |
4-methylumbelliferyl 4-deoxy-beta-D-xylopyranoside | recombinant wild type enzyme, at pH 7.0 and 37°C | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.03 | - |
recombinant wild type enzyme, at pH 7.0 and 37°C | Homo sapiens | 4-methylumbelliferyl 4-deoxy-4-fluoro-beta-D-xylopyranoside | |
0.53 | - |
recombinant wild type enzyme, at pH 7.0 and 37°C | Homo sapiens | 4-methylumbelliferyl 4-deoxy-beta-D-xylopyranoside |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme Y196F, at pH 7.0 and 37°C | Homo sapiens | |
1.2 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme R270A, at pH 7.0 and 37°C | Homo sapiens | |
1.42 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme R270K, at pH 7.0 and 37°C | Homo sapiens | |
1.47 | - |
UDP-alpha-D-galactose | mutant enzyme Y196F, at pH 7.0 and 37°C | Homo sapiens | |
1.87 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme R226A, at pH 7.0 and 37°C | Homo sapiens | |
1.88 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme Y199F, at pH 7.0 and 37°C | Homo sapiens | |
2.32 | - |
UDP-alpha-D-galactose | mutant enzyme R270K, at pH 7.0 and 37°C | Homo sapiens | |
2.85 | - |
UDP-alpha-D-galactose | mutant enzyme R226A, at pH 7.0 and 37°C | Homo sapiens | |
2.92 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme R226K, at pH 7.0 and 37°C | Homo sapiens | |
2.95 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme H195Q, at pH 7.0 and 37°C | Homo sapiens | |
3 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme H195A, at pH 7.0 and 37°C | Homo sapiens | |
3.07 | - |
UDP-alpha-D-galactose | mutant enzyme R270A, at pH 7.0 and 37°C | Homo sapiens | |
4.03 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | mutant enzyme H195R, at pH 7.0 and 37°C | Homo sapiens | |
4.05 | - |
UDP-alpha-D-galactose | mutant enzyme Y199F, at pH 7.0 and 37°C | Homo sapiens | |
4.17 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | recombinant wild type enzyme, at pH 7.0 and 37°C | Homo sapiens | |
4.7 | - |
UDP-alpha-D-galactose | mutant enzyme R226K, at pH 7.0 and 37°C | Homo sapiens | |
4.85 | - |
UDP-alpha-D-galactose | mutant enzyme H195A, at pH 7.0 and 37°C | Homo sapiens | |
4.92 | - |
UDP-alpha-D-galactose | mutant enzyme H195Q, at pH 7.0 and 37°C | Homo sapiens | |
4.92 | - |
UDP-alpha-D-galactose | mutant enzyme H195R, at pH 7.0 and 37°C | Homo sapiens | |
7.08 | - |
UDP-alpha-D-galactose | recombinant wild type enzyme, at pH 7.0 and 37°C | Homo sapiens |