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Literature summary for 2.4.1.38 extracted from

  • Malissard, M.; Borsig, L.; Di Marco, S.; Gruetter, M.G.; Kragl, U.; Wandrey, C.; Berger, E.G.
    Recombinant soluble beta-1,4-galactosyltransferases expressed in Saccharomyces cerevisiae. Purification, characterization and comparison with human enzyme (1996), Eur. J. Biochem., 239, 340-348.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
alpha-lactalbumin stimulates transfer of galactose from UDPgalactose to N-acetylgalactosamine Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
enzymatically active soluble N-deglycosylated enzyme form Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km-value for ovalbumin for the N-deglycosylated recombinant enzyme is 6 mg/ml, and 15 mg/ml for th recombinant enzyme Homo sapiens
0.091
-
UDPgalactose recombinant enzyme Homo sapiens
0.106
-
UDPgalactose
-
Homo sapiens
0.143
-
UDPgalactose recombinant N-deglycosylated enzyme Homo sapiens
0.2
-
UDPgalactose reaction with glucose, wild-type enzyme and N-deglycosylated recombinant enzyme Homo sapiens
0.21
-
UDPgalactose reaction with glucose, recombinant enzyme Homo sapiens
1
-
D-glucose recombinant enzyme Homo sapiens
1.1
-
D-glucose recombinant N-deglycosylated enzyme Homo sapiens
2
-
D-glucose
-
Homo sapiens
3.3
-
N-acetylglucosamine
-
Homo sapiens
8.3
-
N-acetylglucosamine recombinant N-deglycosylated enzyme Homo sapiens
10
-
N-acetylglucosamine recombinant enzyme Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
2 * 55000, SDS-PAGE Homo sapiens
106000
-
calculation from light-scattering experiments Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
milk
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.9
-
N-deglycosylated recombinant enzyme Homo sapiens
8.4
-
recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDPgalactose + glucose in presence of alpha-lactalbumin Homo sapiens lactose + UDP
-
?
UDPgalactose + N-acetylglucosamine
-
Homo sapiens UDP + N-acetyllactosamine
-
?

Subunits

Subunits Comment Organism
dimer 2 * 55000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
4 h, 18% loss of activity Homo sapiens
50
-
1 h, complete loss of activity Homo sapiens