Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
C134S | complete loss of activity | Bos taurus |
C342S | 33fold increase in the apparent Km-value for UDPgalactose | Bos taurus |
additional information | N-terminal truncated forms of the enzyme between residues 1-129, do not show any significant difference in the apparent Km-values towards N-acetylglucosamine or linear oligosaccharide acceptors, e.g. for chitobiose and chitotriose, or for the nucleotide donor UDPgalactose. The binding behaviour of N-terminal and C-terminal fragments of the enzyme towards the N-acetylglucosamine-agarose and UDP-agarose columns differ, the former binds preferentially to the N-acetylglucosamine-columns, while the latter binds to UDP-agarose columns via Mn2+ | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDPgalactose + chitobiose | - |
Bos taurus | ? | - |
? | |
UDPgalactose + chitotriose | - |
Bos taurus | ? | - |
? | |
UDPgalactose + N-acetylglucosamine | - |
Bos taurus | UDP + N-acetyllactosamine | - |
? |