Protein Variants | Comment | Organism |
---|---|---|
S185C | mutant enzyme exhibits 4.8fold elevations in kcat/Km for UDP-glucose relative to that of wild-type enzyme | Homo sapiens |
S185D | activity with UDP-galactose is 0.09% of wild-type activity | Homo sapiens |
S185E | activity with UDP-galactose is 0.04% of wild-type activity | Homo sapiens |
S185N | mutant enzyme exhibits 4.3fold elevations in kcat/Km for UDP-glucose relative to that of wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
UDP-galactose | 37°C, pH 7.0, wild-type enzyme S185C | Homo sapiens | |
0.053 | - |
UDP-galactose | 37°C, pH 7.0, wild-type enzyme | Homo sapiens | |
0.12 | - |
UDP-glucose | 37°C, pH 7.0, wild-type enzyme S185N | Homo sapiens | |
0.152 | - |
UDP-glucose | 37°C, pH 7.0, wild-type enzyme S185C | Homo sapiens | |
0.166 | - |
UDP-galactose | 37°C, pH 7.0, wild-type enzyme S185N | Homo sapiens | |
0.188 | - |
UDP-glucose | 37°C, pH 7.0, wild-type enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P16442 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-galactose + alpha-L-Fucp-(1,2)-beta-D-Galp-O(CH2)7CH3 | - |
Homo sapiens | UDP + alpha-L-Fuc-(1,2)-[alpha-D-Galp-(1,3)-]-beta-D-Galp-O(CH2)7CH3 | - |
? | |
UDP-glucose + Fucalpha1-2Galbeta-O(CH2)7CH3 | wild-type enzyme shows very low activity. Mutants, Ser185Asn and Ser185Cys, exhibit 4.3fold and 4.8fold elevation in kcat/Km for UDP-glucose relative to that of wild-type enzyme | Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00053 | - |
UDP-glucose | 37°C, pH 7.0, wild-type enzyme | Homo sapiens | |
0.0014 | - |
UDP-glucose | 37°C, pH 7.0, wild-type enzyme S185N | Homo sapiens | |
0.002 | - |
UDP-glucose | 37°C, pH 7.0, wild-type enzyme S185C | Homo sapiens | |
0.092 | - |
UDP-galactose | 37°C, pH 7.0, wild-type enzyme S185C | Homo sapiens | |
0.52 | - |
UDP-galactose | 37°C, pH 7.0, wild-type enzyme S185N | Homo sapiens | |
5.2 | - |
UDP-galactose | 37°C, pH 7.0, wild-type enzyme | Homo sapiens |