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Literature summary for 2.4.1.37 extracted from

  • Yazer, M.H.; Denomme, G.A.; Rose, N.L.; Palcic, M.M.
    Amino-acid substitution in the disordered loop of blood group B-glycosyltransferase enzyme causes weak B phenotype (2005), Transfusion, 45, 1178-1182.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli strain BL21 Homo sapiens

Protein Variants

Protein Variants Comment Organism
M186V site-directed mutagenesis, mutation is a naturally occuring polymorphism, amino-acid substitution in the disordered loop of the enzyme causes a weak B phenotype of erythrocytes, mutant enzyme shows reduced activity compared to the wild-type enzyme Homo sapiens
R188S site-directed mutagenesis, nearly inactive mutant Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant M186V enzymes from Escherichia coli by ion exchange and UDP-hexanolamine affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-D-galactose + H-disaccharide synthetic substrate Homo sapiens UDP + alpha-D-galactosyl-1,3-H disaccharide
-
?

Synonyms

Synonyms Comment Organism
blood group B-glycosyltransferase
-
Homo sapiens
GTB
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Homo sapiens
0.000004
-
UDP-D-galactose mutant R188S enzyme Homo sapiens
0.15
-
UDP-D-galactose mutant M186V enzyme Homo sapiens
5.1
-
UDP-D-galactose wild-type enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Homo sapiens