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Literature summary for 2.4.1.37 extracted from

  • Ketcham, C.; Wang, F.; Fisher, S.Z.; Ercan, A.; van der Wel, H.; Locke, R.D.; Sirajud-Doulah, k.; Matta, K.L.; West, C.M.
    Specificity of a soluble UDP-galactose:fucoside alpha1,3-galactosyltransferase that modifies the cytoplasmic glycoprotein Skp1 in Dictyostelium (2004), J. Biol. Chem., 279, 29050-29059.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
DTT required, best at 2 mM Dictyostelium discoideum
additional information not affected by Tween 20 at 0.1-1.0% Dictyostelium discoideum

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition Dictyostelium discoideum
Fucalpha(1,2)Galbeta1-benzyl competitive, 37% and 61% inhibition at 1 mM and 4 mM, respectively Dictyostelium discoideum
additional information not affected by Tween 20 at 0.1-1.0% Dictyostelium discoideum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics, hyperbolic dependence on UDP-Gal concentration Dictyostelium discoideum
0.0035
-
UDP-Gal pH 7.4, 22°C Dictyostelium discoideum
0.006
-
Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-Skp1 protein pH 7.4, 22°C Dictyostelium discoideum
0.82
-
Fucalpha(1,2)Galbeta-1-(4-nitrophenol) pH 7.4, 22°C Dictyostelium discoideum
0.84
-
Fucalpha(1,2)Galbeta1-benzyl pH 7.4, 22°C Dictyostelium discoideum
1.2
-
Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-(4-nitrophenol) pH 7.4, 22°C Dictyostelium discoideum
2.2
-
Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-benzyl pH 7.4, 22°C Dictyostelium discoideum
3.7
-
Fucalpha-1-(4-nitrophenol) pH 7.4, 22°C Dictyostelium discoideum
3.8
-
Fucalpha-1-benzyl pH 7.4, 22°C Dictyostelium discoideum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Dictyostelium discoideum 5737
-
additional information no activity in microsomes Dictyostelium discoideum
-
-
soluble
-
Dictyostelium discoideum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
KCl lower activity compared to NaCl, best at 50 mM Dictyostelium discoideum
Mg2+ 4fold lower activity compared to Mn2+ Dictyostelium discoideum
Mn2+ required, best at 2mM Dictyostelium discoideum
NaCl preferred salt, best at 50 mM Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-D-galactose + fucosylated Skp1 protein Dictyostelium discoideum
-
UDP + alpha-D-galactosyl(1-3)-fucosyl-Skp1 protein
-
?

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum
-
Skp1B overexpressing strain HW302
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 2400fold by 2 steps ion exchange chromatography, HI chromatography, and gel filtration to homogeneity Dictyostelium discoideum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Dictyostelium discoideum
72
-
purified native enzyme Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity, enzyme forms Galalpha1,3Fuc-linkages, specific for L-fucose residues Dictyostelium discoideum ?
-
?
UDP-D-galactose + Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-(4-nitrophenol)
-
Dictyostelium discoideum UDP + alpha-D-Gal-1,3-Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-(4-nitrophenol)
-
?
UDP-D-galactose + Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-benzyl
-
Dictyostelium discoideum UDP + alpha-D-Gal(1,3)Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-benzyl
-
?
UDP-D-galactose + Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-Skp1 protein best substrate, Skp1A1-(HW120)-myc and Skp1 from strain HL250 Dictyostelium discoideum UDP + alpha-D-Gal(1,3)Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-Skp1 protein
-
?
UDP-D-galactose + Fucalpha(1,2)Galbeta-1-(4-nitrophenol)
-
Dictyostelium discoideum UDP + alpha-D-Gal(1,3)Fucalpha(1,2)Galbeta-1-(4-nitrophenol)
-
?
UDP-D-galactose + Fucalpha(1,2)Galbeta-1-benzyl
-
Dictyostelium discoideum UDP + alpha-D-Gal(1,3)Fucalpha(1,2)Galbeta-1-benzyl
-
?
UDP-D-galactose + Fucalpha-1-(4-nitrophenol)
-
Dictyostelium discoideum UDP + alpha-D-Gal(1,3)Fucalpha-1-(4-nitrophenol)
-
?
UDP-D-galactose + Fucalpha-1-benzyl
-
Dictyostelium discoideum UDP + alpha-D-Gal(1,3)Fucalpha-1-benzyl
-
?
UDP-D-galactose + fucosylated Skp1 protein
-
Dictyostelium discoideum UDP + alpha-D-galactosyl(1-3)-fucosyl-Skp1 protein
-
?

Synonyms

Synonyms Comment Organism
alphaGal-T1
-
Dictyostelium discoideum
UDP-galactose:fucoside alpha1,3-galactosyltransferase
-
Dictyostelium discoideum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
-
Dictyostelium discoideum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4 7.4 dependent on the buffer system Dictyostelium discoideum