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Literature summary for 2.4.1.37 extracted from

  • Carne, L.R.; Watkins, W.M.
    Human blood group B gene-specified alpha-3-galactosyltransferase: purification of the enzyme in serum by biospecific adsorption onto blood group O erythrocyte membranes (1977), Biochem. Biophys. Res. Commun., 77, 700-707.
    View publication on PubMed
Search for more literature for 2.4.1.37

Inhibitors

Inhibitors Comment Organism Structure
UDP competitive with respect to UDP-galactose Homo sapiens
UDP-N-acetylgalactosamine weak, competitive with respect to UDP-galactose Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 UDP-galactose
-
 Homo sapiens
0.5
-
 2'-fucosyllactose
-
 Homo sapiens
2.2
-
 O-alpha-L-fucosyl-1,2-galactose
-
 Homo sapiens
2.5
-
 lacto-N-fucopentaose I
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ slight activation Homo sapiens
Mn2+ divalent cation required, Mn2+ most effective, optimum concentration 20 mM Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 blood group B donors
-

Purification (Commentary)

Purification (Comment) Organism
one-step procedure involving absorption onto group 0 erythrocyte membranes followed by elution with the low molecular weight H-active trisaccharide 2'-fucosyllactose Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
 Homo sapiens
-
stomach
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.92
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + 2'-fucosyllactose
-
 Homo sapiens UDP + alpha-D-galactosyl-1,3-[2'-fucosyllactose]
-
 ?
UDP-galactose + glycoprotein alpha-L-fucosyl-1,2-D-galactose enzyme transfers D-galactose in alpha-linkage to oligosaccharides, glycolipids and glycoproteins with terminal non-reducing H-active structures and confers blood group B activity on group 0 erythrocytes Homo sapiens UDP + glycoprotein alpha-D-galactosyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose
-
 ?
UDP-galactose + H-active glycoprotein
-
 Homo sapiens UDP + B-active substance
-
 ?
UDP-galactose + lacto-N-fucopentaose I
-
 Homo sapiens UDP + alpha-D-galactosyl-1,3-[lacto-N-fucopentaose I]
-
 ?
UDP-galactose + O-alpha-L-fucosyl-1,2-galactose
-
 Homo sapiens UDP + alpha-D-galactosyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-galactopyranoside
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 5 min, 20% loss of activity, 20 min, 60% loss of activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Homo sapiens