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Literature summary for 2.4.1.369 extracted from
- In vitro characterization of IroB, a pathogen-associated C-glycosyltransferase (2005), Proc. Natl. Acad. Sci. USA, 102, 571-576 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
enterobactin | pH 7.5, 25°C | Escherichia coli O6:H1 |  |
| 0.013 | - |
UDP-alpha-D-glucose | pH 7.5, 25°C | Escherichia coli O6:H1 | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli O6:H1 | no substrate: N-(2,3-dihydroxybenzoyl)serine | ? | - |
? | |
| additional information | Escherichia coli O6:H1 CFT073 | no substrate: N-(2,3-dihydroxybenzoyl)serine | ? | - |
? | |
| UDP-alpha-D-glucose + diglucosyl-enterobactin | Escherichia coli O6:H1 | - |
UDP + triglucosyl-enterobactin | - |
? | |
| UDP-alpha-D-glucose + diglucosyl-enterobactin | Escherichia coli O6:H1 CFT073 | - |
UDP + triglucosyl-enterobactin | - |
? | |
| UDP-alpha-D-glucose + enterobactin | Escherichia coli O6:H1 | - |
UDP + monoglucosyl-enterobactin | - |
? | |
| UDP-alpha-D-glucose + enterobactin | Escherichia coli O6:H1 CFT073 | - |
UDP + monoglucosyl-enterobactin | - |
? | |
| UDP-alpha-D-glucose + monoglucosyl-enterobactin | Escherichia coli O6:H1 | - |
UDP + diglucosyl-enterobactin | - |
? | |
| UDP-alpha-D-glucose + monoglucosyl-enterobactin | Escherichia coli O6:H1 CFT073 | - |
UDP + diglucosyl-enterobactin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli O6:H1 | A0A0H2V630 | - |
- |
| Escherichia coli O6:H1 CFT073 | A0A0H2V630 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no substrate: N-(2,3-dihydroxybenzoyl)serine | Escherichia coli O6:H1 | ? | - |
? | |
| additional information | no substrate: N-(2,3-dihydroxybenzoyl)serine | Escherichia coli O6:H1 CFT073 | ? | - |
? | |
| UDP-alpha-D-glucose + diglucosyl-enterobactin | - |
Escherichia coli O6:H1 | UDP + triglucosyl-enterobactin | - |
? | |
| UDP-alpha-D-glucose + diglucosyl-enterobactin | - |
Escherichia coli O6:H1 CFT073 | UDP + triglucosyl-enterobactin | - |
? | |
| UDP-alpha-D-glucose + enterobactin | - |
Escherichia coli O6:H1 | UDP + monoglucosyl-enterobactin | - |
? | |
| UDP-alpha-D-glucose + enterobactin | - |
Escherichia coli O6:H1 CFT073 | UDP + monoglucosyl-enterobactin | - |
? | |
| UDP-alpha-D-glucose + monoglucosyl-enterobactin | - |
Escherichia coli O6:H1 | UDP + diglucosyl-enterobactin | - |
? | |
| UDP-alpha-D-glucose + monoglucosyl-enterobactin | - |
Escherichia coli O6:H1 CFT073 | UDP + diglucosyl-enterobactin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| iroB | - |
Escherichia coli O6:H1 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
UDP-alpha-D-glucose | pH 7.5, 25°C | Escherichia coli O6:H1 | |
| 0.18 | - |
enterobactin | pH 7.5, 25°C | Escherichia coli O6:H1 | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | IroB transfers glucosyl groups from uridine-5'-diphosphoglucose to C5 of one, two, or three of the 2,3-dihydroxybenzoyl units of enterobactin to yield monoglucosyl-C-enterobactin, diglucosyl-C-enterobactin, and triglucosyl-C-enterobactin, known as salmochelins | Escherichia coli O6:H1 |
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