Literature summary for 2.4.1.367 extracted from

  • Wei, W.; Wang, P.; Wei, Y.; Liu, Q.; Yang, C.; Zhao, G.; Yue, J.; Yan, X.; Zhou, Z.
    Characterization of Panax ginseng UDP-glycosyltransferases catalyzing protopanaxatriol and biosyntheses of bioactive ginsenosides F1 and Rh1 in metabolically engineered yeasts (2015), Mol. Plant, 8, 1412-1424 .
    View publication on PubMed

Engineering

Protein Variants Comment Organism
G338R the mutant of isoform UGTPg100 is inactive towards (20S)-protopanaxatriol Panax ginseng
A142T the mutant of isoform UGTPg100 is inactive towards (20S)-protopanaxatriol Panax ginseng
L186S the mutant of isoform UGTPg100 is inactive towards (20S)-protopanaxatriol Panax ginseng
W205R the mutant of isoform UGTPg100 remains active with (20S)-protopanaxatriol Panax ginseng

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.153
-
(20S)-protopanaxatriol at pH 8.0 and 35°C Panax ginseng

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + (20S)-protopanaxatriol Panax ginseng
-
UDP + ginsenoside Rh1
-
?
UDP-alpha-D-glucose + ginsenoside F1 Panax ginseng
-
UDP + (20S)-ginsenoside Rg1
-
?

Organism

Organism UniProt Comment Textmining
Panax ginseng A0A0K0PVW1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + (20S)-protopanaxatriol
-
Panax ginseng UDP + ginsenoside Rh1
-
?
UDP-alpha-D-glucose + ginsenoside F1
-
Panax ginseng UDP + (20S)-ginsenoside Rg1
-
?

Synonyms

Synonyms Comment Organism
UGTPg100
-
Panax ginseng

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.1
-
(20S)-protopanaxatriol at pH 8.0 and 35°C Panax ginseng

KCat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
60
-
(20S)-protopanaxatriol at pH 8.0 and 35°C Panax ginseng