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Literature summary for 2.4.1.363 extracted from

  • Wei, W.; Wang, P.; Wei, Y.; Liu, Q.; Yang, C.; Zhao, G.; Yue, J.; Yan, X.; Zhou, Z.
    Characterization of Panax ginseng UDP-glycosyltransferases catalyzing protopanaxatriol and biosyntheses of bioactive ginsenosides F1 and Rh1 in metabolically engineered yeasts (2015), Mol. Plant, 8, 1412-1424 .
    View publication on PubMed
Search for more literature for 2.4.1.363

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Panax ginseng

Protein Variants

Protein Variants Comment Organism
A40S the mutant of isoform UGTPg1 shows about wild type activity towards (20S)-protopanaxatriol Panax ginseng
F85L the mutant of isoform UGTPg1 shows about wild type activity towards (20S)-protopanaxatriol Panax ginseng
H144Y the mutant of isoform UGTPg1 is inactive towards (20S)-protopanaxatriol Panax ginseng
L38F the mutant of isoform UGTPg1 shows about wild type activity towards (20S)-protopanaxatriol Panax ginseng
Q388H the mutant of isoform UGTPg1 shows about wild type activity towards (20S)-protopanaxatriol Panax ginseng
T134I the mutant of isoform UGTPg1 shows about wild type activity towards (20S)-protopanaxatriol Panax ginseng
T142A the mutant of isoform UGTPg103 is inactive towards (20S)-protopanaxatriol Panax ginseng
T142A/S186L the mutant of isoform UGTPg103 is inactive towards (20S)-protopanaxatriol Panax ginseng
T142A/S186L/R338G the mutant of isoform UGTPg103 has activity to glycosylate the C6-OH of (20S)-protopanaxatriol Panax ginseng

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.104
-
 (20S)-protopanaxadiol isoform UGTPg1, at pH 8.0 and 35°C Panax ginseng

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + (20S)-protopanaxadiol Panax ginseng
-
 UDP + ginsenoside C-K
-
 ?

Organism

Organism UniProt Comment Textmining
Panax ginseng
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information isoforms UGTPg102 and UGTPg103 have no detectable activity on (20S)-protopanaxatriol Panax ginseng ?
-
 ?
UDP-alpha-D-glucose + (20S)-protopanaxadiol
-
 Panax ginseng UDP + ginsenoside C-K
-
 ?
UDP-alpha-D-glucose + (20S)-protopanaxadiol the reaction is catalyzed by the enzyme isoform UGTPg1 Panax ginseng UDP + ginsenoside C-K
-
 ?
UDP-alpha-D-glucose + (20S)-protopanaxatriol the reaction is catalyzed by the enzyme isoform UGTPg1 Panax ginseng UDP + ginsenoside F1
-
 ?
UDP-alpha-D-glucose + ginsenoside Rg3 the reaction is catalyzed by the enzyme isoform UGTPg1 Panax ginseng UDP + ginsenoside Rd
-
 ?
UDP-alpha-D-glucose + ginsenoside Rh2 the reaction is catalyzed by the enzyme isoform UGTPg1 Panax ginseng UDP + ginsenoside F2
-
 ?

Synonyms

Synonyms Comment Organism
UGT
-
 Panax ginseng
UGTPg1
-
 Panax ginseng
UGTPg1 isoform Panax ginseng
UGTPg102 isoform Panax ginseng
UGTPg103 isoform Panax ginseng
uridine diphosphate glycosyltransferase
-
 Panax ginseng

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.9
-
 (20S)-protopanaxadiol isoform UGTPg1, at pH 8.0 and 35°C Panax ginseng

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
170
-
 (20S)-protopanaxadiol isoform UGTPg1, at pH 8.0 and 35°C Panax ginseng