Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | 400 mM, 49% inhibition | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
GDP-alpha-D-mannose | pH 7.6, 95°C | Thermotoga maritima | |
16 | - |
di-myo-inositol-1,3'-phosphate | pH 7.6, 95°C | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for maximal activity, 10 mM activates 3.3fold | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-alpha-D-mannose + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate | Thermotoga maritima | the enzyme is involved in the synthesis of the solutes 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate | GDP + 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate | - |
? | |
GDP-alpha-D-mannose + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate | Thermotoga maritima DSM 3109 | the enzyme is involved in the synthesis of the solutes 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate | GDP + 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate | - |
? | |
GDP-alpha-D-mannose + di-myo-inositol-1,3'-phosphate | Thermotoga maritima | the enzyme is involved in the synthesis of the solutes 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate | GDP + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate | - |
? | |
GDP-alpha-D-mannose + di-myo-inositol-1,3'-phosphate | Thermotoga maritima DSM 3109 | the enzyme is involved in the synthesis of the solutes 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate | GDP + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9WYJ4 | - |
- |
Thermotoga maritima DSM 3109 | Q9WYJ4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-alpha-D-mannose + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate | the enzyme is involved in the synthesis of the solutes 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate | Thermotoga maritima | GDP + 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate | - |
? | |
GDP-alpha-D-mannose + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate | minor amounts of the tri-mannosylated form are also detected | Thermotoga maritima | GDP + 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate | - |
? | |
GDP-alpha-D-mannose + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate | the enzyme is involved in the synthesis of the solutes 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate | Thermotoga maritima DSM 3109 | GDP + 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate | - |
? | |
GDP-alpha-D-mannose + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate | minor amounts of the tri-mannosylated form are also detected | Thermotoga maritima DSM 3109 | GDP + 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate | - |
? | |
GDP-alpha-D-mannose + di-myo-inositol-1,3'-phosphate | the enzyme is involved in the synthesis of the solutes 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate | Thermotoga maritima | GDP + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate | - |
? | |
GDP-alpha-D-mannose + di-myo-inositol-1,3'-phosphate | no activity with ADP-glucose, GDP-glucose, UDP-glucose, and TDP-glucose as glycosyl donor | Thermotoga maritima | GDP + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate | - |
? | |
GDP-alpha-D-mannose + di-myo-inositol-1,3'-phosphate | the enzyme is involved in the synthesis of the solutes 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate | Thermotoga maritima DSM 3109 | GDP + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate | - |
? | |
GDP-alpha-D-mannose + di-myo-inositol-1,3'-phosphate | no activity with ADP-glucose, GDP-glucose, UDP-glucose, and TDP-glucose as glycosyl donor | Thermotoga maritima DSM 3109 | GDP + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MDIP synthase | - |
Thermotoga maritima |
TM_0359 | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Thermotoga maritima |
95 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 100 | 80°C: about 40% of maximal activity, 100°C: about 65% of maximal activity | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Thermotoga maritima |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the synthesis of the solutes 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate. In cells grown under heat stress, 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate is the major solute, accounting for 43% of the total pool. 2-O-beta-D-Mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate accumulate to similar levels, each corresponding to 11.5% of the total pool | Thermotoga maritima |