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Literature summary for 2.4.1.360 extracted from

  • Kerscher, F.; Franz, G.
    Isolation and Some Properties of an UDP-glucose 2-hydroxyflavanone-6(or 8)-C-glucosyltransferase from Fagopyrum esculentum M. cotyledons (1988), J. Plant Physiol., 132, 110-115 .
No PubMed abstract available
Search for more literature for 2.4.1.360

Inhibitors

Inhibitors Comment Organism Structure
2,4-dihydroxydibenzoylmethane aglycon concentrations higher than 0.05 mM inhibit the reaction; competitive inhibitor Fagopyrum esculentum
2-hydroxynaringenin aglycon concentrations higher than 0.05 mM inhibit the reaction Fagopyrum esculentum
Fe2+
-
 Fagopyrum esculentum
Mn2+
-
 Fagopyrum esculentum
additional information concentrations of the glucosyldonor up to 0.5 mM increase the rate of product formation. EDTA has no significant effect in activity Fagopyrum esculentum
Zn2+
-
 Fagopyrum esculentum

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ increases the enzyme activity at pH 8 Fagopyrum esculentum
Fe3+ stimulates activity Fagopyrum esculentum
additional information Mg2+, Ca2+, Co2+ and Cu2+ have no significant effect in activity Fagopyrum esculentum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 gel filtration Fagopyrum esculentum

Organism

Organism UniProt Comment Textmining
Fagopyrum esculentum
-
 Moench
-

Purification (Commentary)

Purification (Comment) Organism
-
 Fagopyrum esculentum

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Fagopyrum esculentum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with 2,5-dihydroxyflavanone or 2,4-dihydroxydibenzoylmethane. The enzyme also transfered the sugar moiety from UDP-Gal and UDP-Xyl to the aglycon by forming a C-glycosidic linkage, but not from UDP-GlcA Fagopyrum esculentum ?
-
 ?
UDP-alpha-D-glucose + 2,5,7-trihydroxy-2-phenyl-2,3-dihydro-4H-1-benzopyran-4-one
-
 Fagopyrum esculentum UDP + ?
-
 ?
UDP-alpha-D-glucose + 2-hydroxynaringenin
-
 Fagopyrum esculentum UDP + isovitexin + H2O
-
 ?
UDP-alpha-D-glucose + 2-hydroxynaringenin
-
 Fagopyrum esculentum UDP + vitexin + H2O
-
 ?
UDP-D-galactose + 2-hydroxynaringenin
-
 Fagopyrum esculentum UDP + isovitexin
-
 ?
UDP-D-galactose + 2-hydroxynaringenin
-
 Fagopyrum esculentum UDP + vitexin
-
 ?
UDP-xylose + 2-hydroxynaringenin
-
 Fagopyrum esculentum UDP + isovitexin
-
 ?
UDP-xylose + 2-hydroxynaringenin
-
 Fagopyrum esculentum UDP + vitexin
-
 ?

Synonyms

Synonyms Comment Organism
UDP-glucose:2-hydroxyflavanone-6(or8)-C-glucosyltransferase
-
 Fagopyrum esculentum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.8
-
-
 Fagopyrum esculentum