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Literature summary for 2.4.1.360 extracted from

  • Ito, T.; Fujimoto, S.; Suito, F.; Shimosaka, M.; Taguchi, G.
    C-Glycosyltransferases catalyzing the formation of di-C-glucosyl flavonoids in citrus plants (2017), Plant J., 91, 187-198.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2(DE3) Citrus unshiu
expressed in Escherichia coli Rosetta 2(DE3) Citrus japonica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00085
-
2-hydroxynaringenin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica
0.0144
-
nothofagin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica
0.0715
-
UDP-alpha-D-glucose 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C, nothofagin as sugar acceptor Citrus japonica
0.112
-
6-beta-D-glucopyranosyl-2-hydroxynaringenin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica

Organism

Organism UniProt Comment Textmining
Citrus japonica
-
-
-
Citrus unshiu
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Citrus unshiu
purification via a nickel-affinity column, concentration using Amicon-Ultra-15 Ultracel-10k Citrus japonica

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Citrus japonica
-
leaf
-
Citrus unshiu
-
leaf
-
Citrus japonica
-
additional information detected in all plant organs Citrus unshiu
-
additional information detected in all plant organs Citrus japonica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 UDP-alpha-D-glucose + 2-hydroxynaringenin
-
Citrus unshiu 2 UDP + 6,8-di-(beta-D-glucopyranosyl)-2-hydroxynaringenin
-
?
2 UDP-alpha-D-glucose + 2-hydroxynaringenin
-
Citrus japonica 2 UDP + 6,8-di-(beta-D-glucopyranosyl)-2-hydroxynaringenin
-
?
2 UDP-alpha-D-glucose + phloretin
-
Citrus unshiu 2 UDP + 3',5'-di-(beta-D-glucopyranosyl)phloretin
-
?
2 UDP-alpha-D-glucose + phloretin
-
Citrus japonica 2 UDP + 3',5'-di-(beta-D-glucopyranosyl)phloretin
-
?
additional information no activity with naringenin, apigenin, quercetin, isoliquiritigenin, propyl gallate, and 2',4'dihydroxyacetophenone suggesting that 2',4',6'-trihydroxyacetophenone-like structures are necessary for substrates to be acceptable. Very low activity with phloridzin Citrus unshiu ?
-
?
additional information no activity with naringenin, apigenin, quercetin, isoliquiritigenin, propyl gallate, and 2',4'dihydroxyacetophenone suggesting that 2',4',6'-trihydroxyacetophenone-like structures are necessary for substrates to be acceptable. Very low activity with phloridzin Citrus japonica ?
-
?
UDP-alpha-D-galactose + 2-hydroxypinocembrin low activity with sugar donor UDP-alpha-D-galactose Citrus unshiu UDP + ?
-
?
UDP-alpha-D-galactose + 2-hydroxypinocembrin low activity with sugar donor UDP-alpha-D-galactose Citrus japonica UDP + ?
-
?
UDP-alpha-D-glucose + 2',4',6'-trihydroxyacetophenone
-
Citrus unshiu UDP + ?
-
?
UDP-alpha-D-glucose + 2',4',6'-trihydroxyacetophenone
-
Citrus japonica UDP + ?
-
?
UDP-alpha-D-glucose + 2,4,6-trihydroxybenzaldehyde
-
Citrus unshiu UDP + ?
-
?
UDP-alpha-D-glucose + 2,4,6-trihydroxybenzaldehyde
-
Citrus japonica UDP + ?
-
?
UDP-alpha-D-glucose + 2-phenyl-2',4',6'-trihydroxyacetophenone
-
Citrus unshiu UDP + ?
-
?
UDP-alpha-D-glucose + 2-phenyl-2',4',6'-trihydroxyacetophenone
-
Citrus japonica UDP + ?
-
?
UDP-alpha-D-glucose + maclurin
-
Citrus unshiu UDP + ?
-
?
UDP-alpha-D-glucose + maclurin
-
Citrus japonica UDP + ?
-
?
UDP-alpha-D-glucose + nothofagin
-
Citrus unshiu UDP + ?
-
?
UDP-alpha-D-glucose + nothofagin
-
Citrus japonica UDP + ?
-
?
UDP-alpha-D-xylose + 6-beta-D-glucopyranosyl-2-hydroxynaringenin
-
Citrus unshiu UDP + ?
-
?
UDP-alpha-D-xylose + 6-beta-D-glucopyranosyl-2-hydroxynaringenin
-
Citrus japonica UDP + ?
-
?

Synonyms

Synonyms Comment Organism
CuCGT
-
Citrus unshiu
FcCGT
-
Citrus japonica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Citrus unshiu
50
-
-
Citrus japonica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
inactivation above Citrus unshiu
60
-
activity is significantly retained up to 60°C Citrus japonica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
UDP-alpha-D-glucose 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C, nothofagin as sugar acceptor Citrus japonica
5.3
-
nothofagin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica
5.7
-
6-beta-D-glucopyranosyl-2-hydroxynaringenin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica
7.6
-
2-hydroxynaringenin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica
12
-
phloretin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica

pH Range

pH Minimum pH Maximum Comment Organism
8 11 more than 65% of maximum activity in this range Citrus unshiu
8 11 more than 65% of maximum activity in this range Citrus japonica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0089
-
2-hydroxynaringenin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica
0.037
-
nothofagin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica
0.11
-
UDP-alpha-D-glucose 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C, nothofagin as sugar acceptor Citrus japonica
0.51
-
6-beta-D-glucopyranosyl-2-hydroxynaringenin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica