Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2(DE3) | Citrus unshiu |
expressed in Escherichia coli Rosetta 2(DE3) | Citrus japonica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00085 | - |
2-hydroxynaringenin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica | |
0.0144 | - |
nothofagin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica | |
0.0715 | - |
UDP-alpha-D-glucose | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C, nothofagin as sugar acceptor | Citrus japonica | |
0.112 | - |
6-beta-D-glucopyranosyl-2-hydroxynaringenin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus japonica | - |
- |
- |
Citrus unshiu | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Citrus unshiu |
purification via a nickel-affinity column, concentration using Amicon-Ultra-15 Ultracel-10k | Citrus japonica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Citrus japonica | - |
leaf | - |
Citrus unshiu | - |
leaf | - |
Citrus japonica | - |
additional information | detected in all plant organs | Citrus unshiu | - |
additional information | detected in all plant organs | Citrus japonica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 UDP-alpha-D-glucose + 2-hydroxynaringenin | - |
Citrus unshiu | 2 UDP + 6,8-di-(beta-D-glucopyranosyl)-2-hydroxynaringenin | - |
? | |
2 UDP-alpha-D-glucose + 2-hydroxynaringenin | - |
Citrus japonica | 2 UDP + 6,8-di-(beta-D-glucopyranosyl)-2-hydroxynaringenin | - |
? | |
2 UDP-alpha-D-glucose + phloretin | - |
Citrus unshiu | 2 UDP + 3',5'-di-(beta-D-glucopyranosyl)phloretin | - |
? | |
2 UDP-alpha-D-glucose + phloretin | - |
Citrus japonica | 2 UDP + 3',5'-di-(beta-D-glucopyranosyl)phloretin | - |
? | |
additional information | no activity with naringenin, apigenin, quercetin, isoliquiritigenin, propyl gallate, and 2',4'dihydroxyacetophenone suggesting that 2',4',6'-trihydroxyacetophenone-like structures are necessary for substrates to be acceptable. Very low activity with phloridzin | Citrus unshiu | ? | - |
? | |
additional information | no activity with naringenin, apigenin, quercetin, isoliquiritigenin, propyl gallate, and 2',4'dihydroxyacetophenone suggesting that 2',4',6'-trihydroxyacetophenone-like structures are necessary for substrates to be acceptable. Very low activity with phloridzin | Citrus japonica | ? | - |
? | |
UDP-alpha-D-galactose + 2-hydroxypinocembrin | low activity with sugar donor UDP-alpha-D-galactose | Citrus unshiu | UDP + ? | - |
? | |
UDP-alpha-D-galactose + 2-hydroxypinocembrin | low activity with sugar donor UDP-alpha-D-galactose | Citrus japonica | UDP + ? | - |
? | |
UDP-alpha-D-glucose + 2',4',6'-trihydroxyacetophenone | - |
Citrus unshiu | UDP + ? | - |
? | |
UDP-alpha-D-glucose + 2',4',6'-trihydroxyacetophenone | - |
Citrus japonica | UDP + ? | - |
? | |
UDP-alpha-D-glucose + 2,4,6-trihydroxybenzaldehyde | - |
Citrus unshiu | UDP + ? | - |
? | |
UDP-alpha-D-glucose + 2,4,6-trihydroxybenzaldehyde | - |
Citrus japonica | UDP + ? | - |
? | |
UDP-alpha-D-glucose + 2-phenyl-2',4',6'-trihydroxyacetophenone | - |
Citrus unshiu | UDP + ? | - |
? | |
UDP-alpha-D-glucose + 2-phenyl-2',4',6'-trihydroxyacetophenone | - |
Citrus japonica | UDP + ? | - |
? | |
UDP-alpha-D-glucose + maclurin | - |
Citrus unshiu | UDP + ? | - |
? | |
UDP-alpha-D-glucose + maclurin | - |
Citrus japonica | UDP + ? | - |
? | |
UDP-alpha-D-glucose + nothofagin | - |
Citrus unshiu | UDP + ? | - |
? | |
UDP-alpha-D-glucose + nothofagin | - |
Citrus japonica | UDP + ? | - |
? | |
UDP-alpha-D-xylose + 6-beta-D-glucopyranosyl-2-hydroxynaringenin | - |
Citrus unshiu | UDP + ? | - |
? | |
UDP-alpha-D-xylose + 6-beta-D-glucopyranosyl-2-hydroxynaringenin | - |
Citrus japonica | UDP + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CuCGT | - |
Citrus unshiu |
FcCGT | - |
Citrus japonica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Citrus unshiu |
50 | - |
- |
Citrus japonica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
inactivation above | Citrus unshiu |
60 | - |
activity is significantly retained up to 60°C | Citrus japonica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
UDP-alpha-D-glucose | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C, nothofagin as sugar acceptor | Citrus japonica | |
5.3 | - |
nothofagin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica | |
5.7 | - |
6-beta-D-glucopyranosyl-2-hydroxynaringenin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica | |
7.6 | - |
2-hydroxynaringenin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica | |
12 | - |
phloretin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 11 | more than 65% of maximum activity in this range | Citrus unshiu |
8 | 11 | more than 65% of maximum activity in this range | Citrus japonica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0089 | - |
2-hydroxynaringenin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica | |
0.037 | - |
nothofagin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica | |
0.11 | - |
UDP-alpha-D-glucose | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C, nothofagin as sugar acceptor | Citrus japonica | |
0.51 | - |
6-beta-D-glucopyranosyl-2-hydroxynaringenin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica |