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Literature summary for 2.4.1.358 extracted from

  • Song, C.; Zhao, S.; Hong, X.; Liu, J.; Schulenburg, K.; Schwab, W.
    A UDP-glucosyltransferase functions in both acylphloroglucinol glucoside and anthocyanin biosynthesis in strawberry (Fragaria x ananassa) (2016), Plant J., 85, 730-742 .
    View publication on PubMed
Search for more literature for 2.4.1.358

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Fragaria x ananassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
 1-naphthol pH 7, 30°C, allelic protein UGT71K3b Fragaria x ananassa
0.038
-
 quercetin pH 7, 30°C, allelic protein UGT71K3a Fragaria x ananassa
0.0425
-
 1-naphthol pH 7, 30°C, allelic protein UGT71K3a Fragaria x ananassa
0.065
-
 pelargonidin pH 7, 30°C, allelic protein UGT71K3a Fragaria x ananassa
0.073
-
 pelargonidin pH 7, 30°C, allelic protein UGT71K3b Fragaria x ananassa
0.099
-
 quercetin pH 7, 30°C, allelic protein UGT71K3b Fragaria x ananassa
0.108
-
 kaempferol pH 7, 30°C, allelic protein UGT71K3b Fragaria x ananassa
0.199
-
 kaempferol pH 7, 30°C, allelic protein UGT71K3a Fragaria x ananassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one Fragaria x ananassa the enzyme catalyzes the last step of acylphloroglucinol glucoside biosynthesis UDP + 3,5-dihydroxy-4-(2-methylpropanoyl)phenyl beta-D-glucopyranoside + 3,5-dihydroxy-2-(2-methylpropanoyl)phenyl beta-D-glucopyranoside
-
 ?
UDP-alpha-D-glucose + 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one Fragaria x ananassa the enzyme catalyzes the last step of acylphloroglucinol glucoside biosynthesis UDP + 3,5-dihydroxy-2-(3-methylbutanoyl)phenyl beta-D-glucopyranoside + 3,5-dihydroxy-4-(3-methylbutanoyl)phenyl beta-D-glucopyranoside
-
 ?

Organism

Organism UniProt Comment Textmining
Fragaria x ananassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Fragaria x ananassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information UGT71K3b shows lower activity towards the substrates, except for morin, when compared with the activity of UGT71K3a Fragaria x ananassa ?
-
 ?
UDP-alpha-D-glucose + 1,2,4-benzenetriol poor substrate Fragaria x ananassa UDP + ?
-
 ?
UDP-alpha-D-glucose + 1-naphthol
-
 Fragaria x ananassa UDP + naphthalen-1-yl beta-D-glucopyranoside
-
 ?
UDP-alpha-D-glucose + 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one the enzyme catalyzes the last step of acylphloroglucinol glucoside biosynthesis Fragaria x ananassa UDP + 3,5-dihydroxy-4-(2-methylpropanoyl)phenyl beta-D-glucopyranoside + 3,5-dihydroxy-2-(2-methylpropanoyl)phenyl beta-D-glucopyranoside
-
 ?
UDP-alpha-D-glucose + 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one phlorisovalerophenone i.e. 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one. The 2-glucoside is the major product. More than 80% of the substrate are converted to the corresponding glucoside Fragaria x ananassa UDP + 3,5-dihydroxy-4-(2-methylpropanoyl)phenyl beta-D-glucopyranoside + 3,5-dihydroxy-2-(2-methylpropanoyl)phenyl beta-D-glucopyranoside
-
 ?
UDP-alpha-D-glucose + 2-naphthol both allelic proteins of UGT71K3a/b prefer 2-naphthol and 1-naphthol Fragaria x ananassa UDP + naphthalen-2-yl beta-D-glucopyranoside
-
 ?
UDP-alpha-D-glucose + 3,7-dihydroxyflavone
-
 Fragaria x ananassa UDP + ?
-
 ?
UDP-alpha-D-glucose + 3-hydroxycoumarin
-
 Fragaria x ananassa UDP + 2-oxo-2H-1-benzopyran-3-yl beta-D-glucopyranoside
-
 ?
UDP-alpha-D-glucose + 3-hydroxyflavone
-
 Fragaria x ananassa UDP + 3-[(beta-D-glucopyranosyl)oxy]flavone
-
 ?
UDP-alpha-D-glucose + 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one the enzyme catalyzes the last step of acylphloroglucinol glucoside biosynthesis Fragaria x ananassa UDP + 3,5-dihydroxy-2-(3-methylbutanoyl)phenyl beta-D-glucopyranoside + 3,5-dihydroxy-4-(3-methylbutanoyl)phenyl beta-D-glucopyranoside
-
 ?
UDP-alpha-D-glucose + 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one phlorisobutyrophenone i.e. 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one. The 2-glucoside is the major product. More than 80% of the substrate are converted to the corresponding glucoside Fragaria x ananassa UDP + 3,5-dihydroxy-2-(3-methylbutanoyl)phenyl beta-D-glucopyranoside + 3,5-dihydroxy-4-(3-methylbutanoyl)phenyl beta-D-glucopyranoside
-
 ?
UDP-alpha-D-glucose + 5-hydroxyflavone poor substrate Fragaria x ananassa UDP + 5-[(beta-D-glucopyranosyl)oxy]flavone
-
 ?
UDP-alpha-D-glucose + 6-hydroxycoumarin
-
 Fragaria x ananassa UDP + 2-oxo-2H-1-benzopyran-6-yl beta-D-glucopyranoside
-
 ?
UDP-alpha-D-glucose + 7-hydroxycoumarin
-
 Fragaria x ananassa UDP + 2-oxo-2H-1-benzopyran-7-yl beta-D-glucopyranoside
-
 ?
UDP-alpha-D-glucose + 7-hydroxyflavone
-
 Fragaria x ananassa UDP + 7-[(beta-D-glucopyranosyl)oxy]flavone
-
 ?
UDP-alpha-D-glucose + acetylphloroglucinol
-
 Fragaria x ananassa UDP + 2-acetylphloroglucinol 1-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + apigenin
-
 Fragaria x ananassa UDP + ?
-
 ?
UDP-alpha-D-glucose + catechin
-
 Fragaria x ananassa UDP + ?
-
 ?
UDP-alpha-D-glucose + daphnetin
-
 Fragaria x ananassa UDP + ?
-
 ?
UDP-alpha-D-glucose + epicatechin
-
 Fragaria x ananassa UDP + ?
-
 ?
UDP-alpha-D-glucose + esculetin
-
 Fragaria x ananassa UDP + ?
-
 ?
UDP-alpha-D-glucose + fisetin
-
 Fragaria x ananassa UDP + ?
-
 ?
UDP-alpha-D-glucose + galangin
-
 Fragaria x ananassa UDP + ?
-
 ?
UDP-alpha-D-glucose + isorhamnetin
-
 Fragaria x ananassa UDP + ?
-
 ?
UDP-alpha-D-glucose + kaempferol
-
 Fragaria x ananassa UDP + ?
-
 ?
UDP-alpha-D-glucose + morin
-
 Fragaria x ananassa UDP + ?
-
 ?
UDP-alpha-D-glucose + myricetin
-
 Fragaria x ananassa UDP + ?
-
 ?
UDP-alpha-D-glucose + naringenin
-
 Fragaria x ananassa UDP + ?
-
 ?
UDP-alpha-D-glucose + pelargonidin
-
 Fragaria x ananassa UDP + ?
-
 ?
UDP-alpha-D-glucose + phloroglucinol
-
 Fragaria x ananassa UDP + ?
-
 ?
UDP-alpha-D-glucose + pyrogallol
-
 Fragaria x ananassa UDP + ?
-
 ?
UDP-alpha-D-glucose + quercetin
-
 Fragaria x ananassa UDP + ?
-
 ?
UDP-alpha-D-glucose + scopoletin
-
 Fragaria x ananassa UDP + 6-methoxy-2-oxo-2H-1-benzopyran-7-yl beta-D-glucopyranoside
-
 ?
UDP-alpha-D-glucose + taxifolin
-
 Fragaria x ananassa UDP + ?
-
 ?

Synonyms

Synonyms Comment Organism
UDP-glucose:APG glucosyltransferase
-
 Fragaria x ananassa
UGT71K3
-
 Fragaria x ananassa
UGT71K3a
-
 Fragaria x ananassa
UGT71K3b
-
 Fragaria x ananassa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Fragaria x ananassa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 35 15°C: about 50% of maximal activity, 35°C: about 80% of maximal activity, allelic protein GT71K3a Fragaria x ananassa
15 35 15°C: about 50% of maximal activity, 35°C: about 85% of maximal activity, allelic protein GT71K3b Fragaria x ananassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 allelic protein GT71K3a Fragaria x ananassa
9
-
 allelic protein GT71K3b Fragaria x ananassa

General Information

General Information Comment Organism
malfunction suppression of UGT71K3 activity in transient RNAi-silenced fruits leads to a loss of pigmentation and a substantial decrease of the levels of various APG glucosides and an anthocyanin Fragaria x ananassa
metabolism the enzyme catalyzes the last step of acylphloroglucinol glucoside biosynthesis Fragaria x ananassa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.554
-
 quercetin pH 7, 30°C, allelic protein UGT71K3b Fragaria x ananassa
0.615
-
 kaempferol pH 7, 30°C, allelic protein UGT71K3b Fragaria x ananassa
0.923
-
 kaempferol pH 7, 30°C, allelic protein UGT71K3a Fragaria x ananassa
1.058
-
 1-naphthol pH 7, 30°C, allelic protein UGT71K3a Fragaria x ananassa
1.276
-
 1-naphthol pH 7, 30°C, allelic protein UGT71K3b Fragaria x ananassa
1.38
-
 pelargonidin pH 7, 30°C, allelic protein UGT71K3a Fragaria x ananassa
1.476
-
 pelargonidin pH 7, 30°C, allelic protein UGT71K3b Fragaria x ananassa
1.638
-
 quercetin pH 7, 30°C, allelic protein UGT71K3a Fragaria x ananassa
5.431
-
 acetylphloroglucinol pH 7, 30°C, allelic protein UGT71K3a Fragaria x ananassa