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Literature summary for 2.4.1.357 extracted from

  • Yahyaa, M.; Davidovich-Rikanati, R.; Eyal, Y.; Sheachter, A.; Marzouk, S.; Lewinsohn, E.; Ibdah, M.
    Identification and characterization of UDP-glucose phloretin 4'-O-glycosyltransferase from Malus x domestica Borkh (2016), Phytochemistry, 130, 47-55 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Malus domestica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02611
-
phloretin at pH 7.5 and 30°C Malus domestica
1.19203
-
UDP-alpha-D-glucose at pH 7.5 and 30°C Malus domestica

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+, Mn2+, Ca+ and K+ do not affect enzyme activity Malus domestica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + phloretin Malus domestica 100% activity UDP + trilobatin
-
r

Organism

Organism UniProt Comment Textmining
Malus domestica
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Malus domestica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with UDP-galactose, epicatechin, cyaniding, 4-coumaric acid, and caffeic acid Malus domestica ?
-
?
UDP-alpha-D-glucose + butein 23% activity compared to phloretin Malus domestica UDP + butein-4'-O-glucoside
-
r
UDP-alpha-D-glucose + naringenin 22% activity compared to phloretin Malus domestica UDP + naringenin-7-O-glucoside
-
r
UDP-alpha-D-glucose + phloretin 100% activity Malus domestica UDP + trilobatin
-
r
UDP-alpha-D-glucose + phlorizin 13% activity compared to phloretin Malus domestica UDP + ?
-
r
UDP-alpha-D-glucose + quercetin 20% activity compared to phloretin Malus domestica UDP + quercetin-7-O-glucoside
-
r
UDP-alpha-D-glucose + trilobatin 12% activity compared to phloretin Malus domestica UDP + ?
-
r

Synonyms

Synonyms Comment Organism
Ph-4'-OGT
-
Malus domestica
UDP-glucose:phloretin 4'-O-glycosyltransferase
-
Malus domestica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Malus domestica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 significant activity between 25 and 45°C Malus domestica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0943
-
UDP-alpha-D-glucose at pH 7.5 and 30°C Malus domestica
0.0994
-
phloretin at pH 7.5 and 30°C Malus domestica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Malus domestica

pH Range

pH Minimum pH Maximum Comment Organism
7 9 significant activity at pH 7.0-9.0. Activity decreases to 17% at pH 6.0 and less than 5% at pH 10.0 Malus domestica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.079
-
UDP-alpha-D-glucose at pH 7.5 and 30°C Malus domestica
0.381
-
phloretin at pH 7.5 and 30°C Malus domestica