Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Malus domestica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02611 | - |
phloretin | at pH 7.5 and 30°C | Malus domestica | |
1.19203 | - |
UDP-alpha-D-glucose | at pH 7.5 and 30°C | Malus domestica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mg2+, Mn2+, Ca+ and K+ do not affect enzyme activity | Malus domestica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + phloretin | Malus domestica | 100% activity | UDP + trilobatin | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Malus domestica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with UDP-galactose, epicatechin, cyaniding, 4-coumaric acid, and caffeic acid | Malus domestica | ? | - |
? | |
UDP-alpha-D-glucose + butein | 23% activity compared to phloretin | Malus domestica | UDP + butein-4'-O-glucoside | - |
r | |
UDP-alpha-D-glucose + naringenin | 22% activity compared to phloretin | Malus domestica | UDP + naringenin-7-O-glucoside | - |
r | |
UDP-alpha-D-glucose + phloretin | 100% activity | Malus domestica | UDP + trilobatin | - |
r | |
UDP-alpha-D-glucose + phlorizin | 13% activity compared to phloretin | Malus domestica | UDP + ? | - |
r | |
UDP-alpha-D-glucose + quercetin | 20% activity compared to phloretin | Malus domestica | UDP + quercetin-7-O-glucoside | - |
r | |
UDP-alpha-D-glucose + trilobatin | 12% activity compared to phloretin | Malus domestica | UDP + ? | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Ph-4'-OGT | - |
Malus domestica |
UDP-glucose:phloretin 4'-O-glycosyltransferase | - |
Malus domestica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Malus domestica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | significant activity between 25 and 45°C | Malus domestica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0943 | - |
UDP-alpha-D-glucose | at pH 7.5 and 30°C | Malus domestica | |
0.0994 | - |
phloretin | at pH 7.5 and 30°C | Malus domestica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Malus domestica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | significant activity at pH 7.0-9.0. Activity decreases to 17% at pH 6.0 and less than 5% at pH 10.0 | Malus domestica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.079 | - |
UDP-alpha-D-glucose | at pH 7.5 and 30°C | Malus domestica | |
0.381 | - |
phloretin | at pH 7.5 and 30°C | Malus domestica |