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Literature summary for 2.4.1.357 extracted from
- Isolation and characterization of a novel glycosyltransferase that converts phloretin to phlorizin, a potent antioxidant in apple (2008), FEBS J., 275, 3804-3814 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21-Codon-Plus-RIL cells | Malus domestica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
UDP-alpha-D-glucose | at pH 7.5 and 30°C | Malus domestica |  |
| 0.62 | - |
phloretin | at pH 7.5 and 30°C | Malus domestica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme activity is not affected by the addition of divalent cations (Mg2+ or Mn2+, 0-25 mM) or monovalent cations (Na+, 0-100 mM or K+, 0-10 mM) | Malus domestica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + phloretin | Malus domestica | - |
UDP + phlorizin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Malus domestica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-Trap column chromatography and Superdex 200 gel filtration | Malus domestica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bud | - |
Malus domestica | - |
| flower | highest expression | Malus domestica | - |
| fruit | - |
Malus domestica | - |
| leaf | - |
Malus domestica | - |
| phloem | - |
Malus domestica | - |
| root | - |
Malus domestica | - |
| seed | - |
Malus domestica | - |
| shoot | - |
Malus domestica | - |
| xylem | - |
Malus domestica | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with caffeic acid, catechin, chlorogenic acid, 2-coumaric acid, 3-coumaric acid, 4-coumaric acid, cyanidin, 3-3,4 dihydroxyphenyl propionic acid, epicatechin, 3-hydroxybenzoic acid, naringenin, protocatechuic acid, quercetin and rutin | Malus domestica | ? | - |
? | |
| UDP-alpha-D-galactose + phloretin | - |
Malus domestica | ? | - |
? | |
| UDP-alpha-D-glucose + phloretin | - |
Malus domestica | UDP + phlorizin | - |
? | |
| UDP-alpha-D-xylose + phloretin | - |
Malus domestica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53500, calculated from amino acid sequence | Malus domestica |
| ? | x * 59000, His6-tagged enzyme, SDS-PAGE | Malus domestica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PGT1 | - |
Malus domestica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Malus domestica |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 50 | - |
Malus domestica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0972 | - |
phloretin | at pH 7.5 and 30°C | Malus domestica | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
- |
Malus domestica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | the enzyme shows significant activity from pH 7.0-8.0. Activity decreases to 20% at pH 6.0 and 9.0 and less than 5% at pH 5.0 and 10.0, respectively | Malus domestica |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Malus domestica | calculated from amino acid sequence | - |
5.8 |
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