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Literature summary for 2.4.1.347 extracted from

  • Pan, Y.; Carroll, J.; Elbein, A.
    Trehalose-phosphate synthase of Mycobacterium tuberculosis Cloning, expression and properties of the recombinant enzyme (2002), Eur. J. Biochem., 269, 6091-6100 .
    View publication on PubMed
Search for more literature for 2.4.1.347

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4
-
 D-glucose 6-phosphate with GDP-alpha-D-glucose as cosubstrate, at pH 8.0 and 37°C Mycobacterium tuberculosis
7
-
 D-glucose 6-phosphate with UDP-alpha-D-glucose as cosubstrate, at pH 8.0 and 37°C Mycobacterium tuberculosis
16
-
 GDP-alpha-D-glucose at pH 8.0 and 37°C Mycobacterium tuberculosis
18
-
 UDP-alpha-D-glucose at pH 8.0 and 37°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ enzyme activity is increased about 2fold by 10 mM Mn2+ Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WN11
-
-
Mycobacterium tuberculosis H37Rv P9WN11
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA His-binding resin column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-alpha-D-glucose + D-glucose 6-phosphate ADP-alpha-D-glucose is the best substrate Mycobacterium tuberculosis ADP + alpha,alpha-trehalose 6-phosphate
-
 ?
ADP-alpha-D-glucose + D-glucose 6-phosphate ADP-alpha-D-glucose is the best substrate Mycobacterium tuberculosis H37Rv ADP + alpha,alpha-trehalose 6-phosphate
-
 ?
CDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Mycobacterium tuberculosis CDP + alpha,alpha-trehalose 6-phosphate
-
 ?
CDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Mycobacterium tuberculosis H37Rv CDP + alpha,alpha-trehalose 6-phosphate
-
 ?
GDP-alpha-D-glucose + D-glucose 6-phosphate second best substrate Mycobacterium tuberculosis GDP + alpha,alpha-trehalose 6-phosphate
-
 ?
GDP-alpha-D-glucose + D-glucose 6-phosphate second best substrate Mycobacterium tuberculosis H37Rv GDP + alpha,alpha-trehalose 6-phosphate
-
 ?
TDP-alpha-D-glucose + D-glucose 6-phosphate TDP-alpha-D-glucose is the weakest substrate Mycobacterium tuberculosis TDP + alpha,alpha-trehalose 6-phosphate
-
 ?
TDP-alpha-D-glucose + D-glucose 6-phosphate TDP-alpha-D-glucose is the weakest substrate Mycobacterium tuberculosis H37Rv TDP + alpha,alpha-trehalose 6-phosphate
-
 ?
UDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Mycobacterium tuberculosis UDP + alpha,alpha-trehalose 6-phosphate
-
 ?
UDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Mycobacterium tuberculosis H37Rv UDP + alpha,alpha-trehalose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 58000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
OtsA
-
 Mycobacterium tuberculosis
TPS
-
 Mycobacterium tuberculosis