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Literature summary for 2.4.1.344 extracted from

  • Cheng, P.W.; DeVries, A.
    Mucin biosynthesis. Enzymic properties of human-tracheal epithelial GDP-L-fucose:beta-D-galactoside alpha-(1->2)-L-fucosyltransferase (1986), Carbohydr. Res., 149, 253-261.
    View publication on PubMed
Search for more literature for 2.4.1.344

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 maximum activity at 0.05%. Inhibitory above 0.2% Homo sapiens
Triton X-100 maximum activity at 0.5%, inhibitory above 2% Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 25 mM, 1% residual activity Homo sapiens
Co2+ 25 mM, 1% residual activity Homo sapiens
Fe2+ 25 mM, about 40% residual activity Homo sapiens
GDP 10 mM, almost complete inhibition Homo sapiens
GMP 10 mM, almost complete inhibition Homo sapiens
GTP 10 mM, almost complete inhibition Homo sapiens
IDP 10 mM, 60-75% inhibition Homo sapiens
IMP 10 mM, 60-75% inhibition Homo sapiens
ITP 10 mM, 60-75% inhibition Homo sapiens
N-ethylmaleimide 10 mM, almost complete inhibition Homo sapiens
UTP 10 mM, 30-32% inhibition Homo sapiens
XDP 10 mM, 60-75% inhibition Homo sapiens
XMP 10 mM, 30-32% inhibition Homo sapiens
Zn2+ 25 mM, 1% residual activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
 GDP-beta-L-fucose pH 6.5, 37°C Homo sapiens
5.7
-
 freezing point-depressing glycoprotein pH 6.5, 37°C Homo sapiens
25.4
-
 phenyl beta-D-galactopyranoside pH 6.5, 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ about 2-2.5fold activation Homo sapiens
Ca2+ or Mn2+, about 3fold activation, maximum activity at 20-30 mM Homo sapiens
Mg2+ about 2-2.5fold activation Homo sapiens
Mn2+ or Ca2+, about 3fold activation, maximum activity at 20-30 mM Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
trachea
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R
-
 Homo sapiens GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R
-
 ?
GDP-beta-L-fucose + freezing point-depressing glycoprotein
-
 Homo sapiens GDP + alpha-L-fucosyl-freezing point-depressing glycoprotein
-
 ?
GDP-beta-L-fucose + phenyl beta-D-galactopyranoside
-
 Homo sapiens GDP + phenyl alpha-L-fucosyl-(1->2)-beta-D-galactopyranoside
-
 ?
additional information enzyme transfers L-fucose from GDP-L-fucose to an acceptor containing a beta-D-galactopyranosyl group at the nonreducing terminal Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
GDP-L-fucose:beta-D-galactoside alpha-(1->2)-L-fucosyltransferase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Homo sapiens