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Literature summary for 2.4.1.342 extracted from

  • Koliwer-Brandl, H.; Syson, K.; van de Weerd, R.; Chandra, G.; Appelmelk, B.; Alber, M.; Ioerger, T.; Jacobs, W.J.; Geurtsen, J.; Bornemann, S.; Kalscheuer, R.
    Metabolic network for the biosynthesis of intra- and extracellular alpha-glucans required for virulence of Mycobacterium tuberculosis (2016), PLoS Pathog., 12, e1005768.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.342

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Mycobaterium smegmatis mc2155 Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
alpha-D-glucose-1-phosphate substrate inhibition Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.046
-
 ADP-alpha-D-glucose with 4.0 mM alpha-D-glucose-1-phosphate, at pH 7.0 and 37°C Mycobacterium tuberculosis
0.063
-
 ADP-alpha-D-glucose with 2.5 mM alpha-D-glucose-1-phosphate, at pH 7.0 and 37°C Mycobacterium tuberculosis
0.121
-
 ADP-alpha-D-glucose with 1.0 mM alpha-D-glucose-1-phosphate, at pH 7.0 and 37°C Mycobacterium tuberculosis
0.145
-
 ADP-alpha-D-glucose with 0.25 mM alpha-D-glucose-1-phosphate, at pH 7.0 and 37°C Mycobacterium tuberculosis
0.578
-
 alpha-D-glucose-1-phosphate with 0.5 mM ADP-alpha-D-glucose, at pH 7.0 and 37°C Mycobacterium tuberculosis
0.695
-
 alpha-D-glucose-1-phosphate with 4.0 mM ADP-alpha-D-glucose, at pH 7.0 and 37°C Mycobacterium tuberculosis
0.816
-
 alpha-D-glucose-1-phosphate with 0.1 mM ADP-alpha-D-glucose, at pH 7.0 and 37°C Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP-alpha-D-glucose + alpha-D-glucose-1-phosphate Mycobacterium tuberculosis
-
 ADP + alpha-maltose-1-phosphate
-
 ?
ADP-alpha-D-glucose + alpha-D-glucose-1-phosphate Mycobacterium tuberculosis H37Rv
-
 ADP + alpha-maltose-1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WMZ1
-
-
Mycobacterium tuberculosis H37Rv P9WMZ1
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-alpha-D-glucose + alpha-D-glucose-1-phosphate
-
 Mycobacterium tuberculosis ADP + alpha-maltose-1-phosphate
-
 ?
ADP-alpha-D-glucose + alpha-D-glucose-1-phosphate
-
 Mycobacterium tuberculosis H37Rv ADP + alpha-maltose-1-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
glgA
-
 Mycobacterium tuberculosis
glgM
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.4
-
 ADP-alpha-D-glucose with 4.0 mM alpha-D-glucose-1-phosphate, at pH 7.0 and 37°C Mycobacterium tuberculosis
19.8
-
 ADP-alpha-D-glucose with 2.5 mM alpha-D-glucose-1-phosphate, at pH 7.0 and 37°C Mycobacterium tuberculosis
20
-
 ADP-alpha-D-glucose with 0.25 mM alpha-D-glucose-1-phosphate, at pH 7.0 and 37°C Mycobacterium tuberculosis
29.2
-
 ADP-alpha-D-glucose with 1.0 mM alpha-D-glucose-1-phosphate, at pH 7.0 and 37°C Mycobacterium tuberculosis
39
-
 alpha-D-glucose-1-phosphate with 0.1 mM ADP-alpha-D-glucose, at pH 7.0 and 37°C Mycobacterium tuberculosis
80.6
-
 alpha-D-glucose-1-phosphate with 4.0 mM ADP-alpha-D-glucose, at pH 7.0 and 37°C Mycobacterium tuberculosis
97.9
-
 alpha-D-glucose-1-phosphate with 0.5 mM ADP-alpha-D-glucose, at pH 7.0 and 37°C Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.578
-
 alpha-D-glucose-1-phosphate with 0.5 mM ADP-alpha-D-glucose, at pH 7.0 and 37°C Mycobacterium tuberculosis
0.895
-
 alpha-D-glucose-1-phosphate with 4.0 mM ADP-alpha-D-glucose, at pH 7.0 and 37°C Mycobacterium tuberculosis
1.45
-
 alpha-D-glucose-1-phosphate with 0.1 mM ADP-alpha-D-glucose, at pH 7.0 and 37°C Mycobacterium tuberculosis