Literature summary for 2.4.1.34 extracted from

Brownfield, L.; Wilson, S.; Newbigin, E.; Bacic, A.; Read, S.
Molecular control of the glucan synthase-like protein NaGSL1 and callose synthesis during growth of Nicotiana alata pollen tubes (2008), Biochem. J., 414, 43-52.

Search for more literature for 2.4.1.34

Activating Compound

Activating Compound	Comment	Organism	Structure
CHAPS	pollen CalS isozyme is activated in vitro by the detergent CHAPS, but activation is not associated with a detectable change in the molecular mass of the NaGSL1 polypeptide	Nicotiana alata
additional information	the somatic isozyme is activated by wounding	Nicotiana alata
Trypsin	pollen CalS isozyme, in contrast to the somatic isozyme, is activated in vitro by the proteolytic enzyme trypsin, but activation is not associated with a detectable change in the molecular mass of the NaGSL1 polypeptide. Some CalS activity is lost with longer incubations with trypsin	Nicotiana alata

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	the somatic isozyme is inactivated by trypsin, in contrast to the pollen isozyme	Nicotiana alata

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	inactive, latent enzyme form	Nicotiana alata	5783	-
Golgi membrane	inactive, latent enzyme form	Nicotiana alata	139	-
additional information	predominantly in the endoplasmic reticulum and Golgi membranes in younger pollen tubes mostly in an inactive, latent enzyme form, the latent and active enzyme forms together In later stages of pollen-tube growth to a greater proportion located in intracellular vesicles and the plasma membrane	Nicotiana alata	-	-
plasma membrane	inactive, latent enzyme form and highly active enzyme form, Ca2+-independent developmentally regulated CalS	Nicotiana alata	5886	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	x * 28000, pollen isozyme, SDS-PAGE	Nicotiana alata
220000	-	pollen isozyme	Nicotiana alata

Organism

Organism	UniProt	Comment	Textmining
Nicotiana alata	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	Nicotiana alata contains two CalS isozymes, one is the Ca2+-independent, trypsin activable pollen-tube CalS, the other is the wound-activated somatic cell CalS, which is Ca2+-dependent and generally inactivated by trypsin	Nicotiana alata	-
pollen	pollen isozyme: predominantly in the endoplasmic reticulum and Golgi membranes in younger pollen tubes mostly in an inactive, latent enzyme form, the latent and active enzyme forms together In later stages of pollen-tube growth to a greater proportion located in intracellular vesicles and the plasma membrane. No activity in mature pollen	Nicotiana alata	-
somatic cell	somatic isozyme	Nicotiana alata	-

Subunits

Subunits	Comment	Organism
polymer	x * 28000, pollen isozyme, SDS-PAGE	Nicotiana alata

Synonyms

Synonyms	Comment	Organism
callose synthase	-	Nicotiana alata
CalS	-	Nicotiana alata
glucan synthase-like 1	-	Nicotiana alata
GSL1	-	Nicotiana alata
uridine-diphosphate glucose:1,3-beta-D-glucosyltransferase	-	Nicotiana alata

General Information

General Information	Comment	Organism
physiological function	the active enzyme in the plasma membrane is reponsible for callose synthesis	Nicotiana alata

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Release 2023.1 (January 2023)