Crystallization (Comment) | Organism |
---|---|
two distinct N- and C-terminal halves, similar in size and topology and separated by a deep cleft, both domains contain a similar core structure of parallel beta sheets connected by alpha helices, the C-terminal structure contains a glycine fingerprint sequence | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
D13A | lower turnover and lower Km than wild-type | Staphylococcus aureus |
D332A | lower turnover and higher Km than wild-type | Staphylococcus aureus |
H125A | higher turnover and lower Km than wild-type | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
UDPglucose | H125A | Staphylococcus aureus | |
1.1 | - |
UDPglucose | mutant D13A | Staphylococcus aureus | |
1.3 | - |
UDPglucose | wild-type | Staphylococcus aureus | |
3.3 | - |
UDPglucose | mutant D332A | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDPglucose + (1,3-beta-D-glucosyl)n | - |
Staphylococcus aureus | UDP + (1,3-beta-D-glucosyl)n+1 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00217 | - |
UDPglucose | D332A | Staphylococcus aureus | |
0.0433 | - |
UDPglucose | D13A | Staphylococcus aureus | |
0.55 | - |
UDPglucose | wild-type | Staphylococcus aureus | |
0.717 | - |
UDPglucose | H125A | Staphylococcus aureus |