Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and Streptomyces lividans | Streptomyces hygroscopicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
- |
Streptomyces hygroscopicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + validoxylamine A | Streptomyces hygroscopicus | the role of valG in the glycosylation of validoxylamine to validamycin A is demonstrated in vitro by enzymatic assay | UDP + validamycin A | - |
? | |
UDP-alpha-D-glucose + validoxylamine A | Streptomyces hygroscopicus 5008 | the role of valG in the glycosylation of validoxylamine to validamycin A is demonstrated in vitro by enzymatic assay | UDP + validamycin A | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces hygroscopicus | - |
- |
- |
Streptomyces hygroscopicus 5008 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His6-Tagged ValG | Streptomyces hygroscopicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-alpha-D-glucose + validoxylamine A | UDP-glucose is the most efficient glycosyl donor. Whereas GDP-glucose is less efficient, ADP-glucose iss much less efficient and TDP-glucose is the least efficient donor | Streptomyces hygroscopicus | ADP + validamycin A | - |
? | |
ADP-alpha-D-glucose + validoxylamine A | UDP-glucose is the most efficient glycosyl donor. Whereas GDP-glucose is less efficient, ADP-glucose iss much less efficient and TDP-glucose is the least efficient donor | Streptomyces hygroscopicus 5008 | ADP + validamycin A | - |
? | |
GDP-alpha-D-glucose + validoxylamine A | UDP-glucose is the most efficient glycosyl donor. Whereas GDP-glucose is less efficient, ADP-glucose iss much less efficient and TDP-glucose is the least efficient donor | Streptomyces hygroscopicus | GDP + validamycin A | - |
? | |
GDP-alpha-D-glucose + validoxylamine A | UDP-glucose is the most efficient glycosyl donor. Whereas GDP-glucose is less efficient, ADP-glucose iss much less efficient and TDP-glucose is the least efficient donor | Streptomyces hygroscopicus 5008 | GDP + validamycin A | - |
? | |
TDP-alpha-D-glucose + validoxylamine A | UDP-glucose is the most efficient glycosyl donor. Whereas GDP-glucose is less efficient, ADP-glucose iss much less efficient and TDP-glucose is the least efficient donor | Streptomyces hygroscopicus | TDP + validamycin A | - |
? | |
TDP-alpha-D-glucose + validoxylamine A | UDP-glucose is the most efficient glycosyl donor. Whereas GDP-glucose is less efficient, ADP-glucose iss much less efficient and TDP-glucose is the least efficient donor | Streptomyces hygroscopicus 5008 | TDP + validamycin A | - |
? | |
UDP-alpha-D-glucose + validoxylamine A | the role of valG in the glycosylation of validoxylamine to validamycin A is demonstrated in vitro by enzymatic assay | Streptomyces hygroscopicus | UDP + validamycin A | - |
? | |
UDP-alpha-D-glucose + validoxylamine A | UDP-glucose is the most efficient glycosyl donor. Whereas GDP-glucose is less efficient, ADP-glucose iss much less efficient and TDP-glucose is the least efficient donor | Streptomyces hygroscopicus | UDP + validamycin A | - |
? | |
UDP-alpha-D-glucose + validoxylamine A | the role of valG in the glycosylation of validoxylamine to validamycin A is demonstrated in vitro by enzymatic assay | Streptomyces hygroscopicus 5008 | UDP + validamycin A | - |
? | |
UDP-alpha-D-glucose + validoxylamine A | UDP-glucose is the most efficient glycosyl donor. Whereas GDP-glucose is less efficient, ADP-glucose iss much less efficient and TDP-glucose is the least efficient donor | Streptomyces hygroscopicus 5008 | UDP + validamycin A | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, polyhistidine-tagged protein, SDS-PAGE | Streptomyces hygroscopicus |
Synonyms | Comment | Organism |
---|---|---|
valG | - |
Streptomyces hygroscopicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces hygroscopicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Streptomyces hygroscopicus |
General Information | Comment | Organism |
---|---|---|
malfunction | in vivo inactivation of the putative glycosyltransferase gene (valG) abolished the final attachment of glucose for validamycin A production and results in accumulation of the VAL-A precursor, validoxylamine | Streptomyces hygroscopicus |
metabolism | the enzyme is involved in biosynthesis of validamycin A, a weakly basic C7N-aminocyclitol-containing antibiotic | Streptomyces hygroscopicus |