Literature summary for 2.4.1.329 extracted from

Tauzin, A.; Bruel, L.; Laville, E.; Nicoletti, C.; Navarro, D.; Henrissat, B.; Perrier, J.; Potocki-Veronese, G.; Giardina, T.; Lafond, M.
Sucrose 6F-phosphate phosphorylase A novel insight in the human gut microbiome (2019), Microb. Genom., 5, 1-14 .

Search for more literature for 2.4.1.329

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	[Ruminococcus] gnavus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.7	-	sucrose 6F-phosphate	pH 6, 40°C	[Ruminococcus] gnavus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	gel filtration	[Ruminococcus] gnavus

Organism

Organism	UniProt	Comment	Textmining
[Ruminococcus] gnavus	G4T4R6	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate	-	[Ruminococcus] gnavus	sucrose 6F-phosphate + phosphate	-	r
additional information	enzyme displays selective activity on sucrose 6F-phosphate acting both in phosphorolysis releasing alpha-D-glucose-1-phosphate and alpha-D-fructose-6-phosphate, and in reverse phosphorolysis from alpha-D-glucose-1-phosphate and alpha-D-fructose-6-phosphate to sucrose 6F-phosphate. No substrates: sucrose, D-fructose, D-glucose, D-galalctose, D-mannose, D-N-acetylgalactosamine, D-xylose, maltotetraose, maltoheptaose	[Ruminococcus] gnavus	?	-	-
sucrose 6F-phosphate + phosphate	-	[Ruminococcus] gnavus	alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate	-	r

Subunits

Subunits	Comment	Organism
monomer	1 * 55000, SDS-PAGE, 1 * 55165, calculated from sequence	[Ruminococcus] gnavus

Synonyms

Synonyms	Comment	Organism
RUGNEv3_61221	locus name	[Ruminococcus] gnavus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
740	-	sucrose 6F-phosphate	pH 6, 40°C	[Ruminococcus] gnavus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
435.3	-	sucrose 6F-phosphate	pH 6, 40°C	[Ruminococcus] gnavus

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Release 2023.1 (January 2023)