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Literature summary for 2.4.1.326 extracted from

  • Lu, W.; Leimkuhler, C.; Gatto, G.J. Jr.
    Kruger, R.G.; Oberthür, M.; Kahne, D.; Walsh, C.T.: AknT is an activating protein for the glycosyltransferase AknS in L-aminodeoxysugar transfer to the aglycone of aclacinomycin A (2005), Chem. Biol., 12, 527-534.
    View publication on PubMed
Search for more literature for 2.4.1.326

Activating Compound

Activating Compound Comment Organism Structure
AKnT protein the enzyme AknS has very low activity in the absence of the adjacently encoded AknT activator protein. At a 3:1 ratio in the 0.0025-0.01 mM range, AknT stimulates AknS kcat by 40fold for transfer of L-2-deoxyfucose to aklavinone Streptomyces galilaeus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces galilaeus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0031
-
 aklavinone with dTDP-4-amino-2-deoxyrhamnose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
0.0035
-
 aklavinone with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
0.046
-
 epsilon-rhodomycinone with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
0.156
-
 dTDP-L-2-deoxyfucose with epsilon-rhodomycinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
0.2878
-
 dTDP-L-2-deoxyfucose with aklavinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
0.4072
-
 dTDP-4-amino-2-deoxyrhamnose in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-beta-L-rhodosamine + aklavinone Streptomyces galilaeus
-
 dTDP + aclacinomycin T
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces galilaeus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography, and gel filtration Streptomyces galilaeus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-4-amino-2-deoxyrhamnose + aklavinone
-
 Streptomyces galilaeus dTDP + 4-O-(2,4-dideoxy-4-amino-alpha-L-rhamnopyranosyl)aklavinone
-
 ?
dTDP-4-amino-2-deoxyrhamnose + aklavinone
-
 Streptomyces galilaeus dTDP + 4-O-(2,4-dideoxy-4-amino-alpha-L-rhamnosyl)aklavinone
-
 ?
dTDP-beta-L-rhodosamine + aklavinone
-
 Streptomyces galilaeus dTDP + aclacinomycin T
-
 ?
dTDP-L-2-deoxyfucose + aklavinone
-
 Streptomyces galilaeus dTDP + 4-O-(2-deoxy-alpha-L-fucosyl)aklavinone
-
 ?
dTDP-L-2-deoxyfucose + epsilon-rhodomycinone
-
 Streptomyces galilaeus dTDP + 4-O-(2-deoxy-alpha-L-fucosyl)-epsilon-rhodomycinone
-
 ?

Synonyms

Synonyms Comment Organism
AknS
-
 Streptomyces galilaeus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 epsilon-rhodomycinone with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
0.001
-
 dTDP-L-2-deoxyfucose with epsilon-rhodomycinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
0.003
-
 dTDP-4-amino-2-deoxyrhamnose in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
0.003
-
 aklavinone with dTDP-4-amino-2-deoxyrhamnose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
0.004
-
 dTDP-L-2-deoxyfucose with aklavinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
0.004
-
 aklavinone with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus