Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Xanthomonas campestris pv. campestris |
expressed in Escherichia coli | Neurospora crassa |
expression in Escherichia coli as a His-tagged fusion protein | Neurospora crassa |
expression in Escherichia coli as a His-tagged fusion protein | Xanthomonas campestris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-O-beta-D-glucopyranosyl-D-gluconate | - |
Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
phosphate | pH 7.0, 30°C | Neurospora crassa | |
0.21 | - |
alpha-D-glucose 1-phosphate | pH 6.5, 30°C | Xanthomonas campestris | |
0.21 | - |
alpha-D-glucose 1-phosphate | pH 6.0-6.5, 30°C | Xanthomonas campestris pv. campestris | |
1 | - |
4-O-beta-D-glucopyranosyl-D-gluconate | pH 7.0, 30°C | Neurospora crassa | |
2 | - |
D-gluconate | pH 6.5, 30°C | Xanthomonas campestris | |
2 | - |
D-gluconate | pH 6.0-6.5, 30°C | Xanthomonas campestris pv. campestris | |
2.4 | - |
D-glucuronate | pH 6.5, 30°C | Xanthomonas campestris | |
2.4 | - |
D-glucuronate | pH 6.0-6.5, 30°C | Xanthomonas campestris pv. campestris | |
3.4 | - |
D-gluconate | pH 6.5, 30°C | Neurospora crassa | |
3.4 | - |
D-gluconate | pH 6.0-6.5, 30°C | Neurospora crassa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Xanthomonas campestris pv. campestris | 5737 | - |
cytoplasm | - |
Neurospora crassa | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84000 | - |
2 * 84000, SDS-PAGE | Xanthomonas campestris |
84000 | - |
2 * 84000, SDS-PAGE, 2 * 89700, amino acid sequence | Xanthomonas campestris pv. campestris |
87000 | - |
2 * 87000, SDS-PAGE | Neurospora crassa |
87000 | - |
2 * 87000, SDS-PAGE, 2 * 90796, amino acid sequence | Neurospora crassa |
89700 | - |
2 * 84000, SDS-PAGE, 2 * 89700, amino acid sequence | Xanthomonas campestris pv. campestris |
89700 | - |
2 * 89700, calculated from sequence | Xanthomonas campestris |
90769 | - |
2 * 90769, calculated from sequence | Neurospora crassa |
90796 | - |
2 * 87000, SDS-PAGE, 2 * 90796, amino acid sequence | Neurospora crassa |
171000 | - |
gel filtration | Neurospora crassa |
179000 | - |
gel filtration | Xanthomonas campestris pv. campestris |
179000 | - |
gel filtration | Xanthomonas campestris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | Neurospora crassa | - |
alpha-D-glucose 1 phosphate + D-gluconate | - |
r | |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | Xanthomonas campestris pv. campestris | - |
alpha-D-glucose 1-phosphate + D-gluconate | - |
r | |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | Neurospora crassa | it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively | alpha-D-glucose 1-phosphate + D-gluconate | - |
r | |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | Xanthomonas campestris | it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively | alpha-D-glucose 1-phosphate + D-gluconate | - |
r | |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | Neurospora crassa DSM 1257 | - |
alpha-D-glucose 1 phosphate + D-gluconate | - |
r | |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | Neurospora crassa DSM 1257 | it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively | alpha-D-glucose 1-phosphate + D-gluconate | - |
r | |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | Xanthomonas campestris pv. campestris ATCC 33913 | - |
alpha-D-glucose 1-phosphate + D-gluconate | - |
r | |
alpha-D-glucose 1 phosphate + D-gluconate | Neurospora crassa | - |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | - |
r | |
alpha-D-glucose 1 phosphate + D-gluconate | Neurospora crassa DSM 1257 | - |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + D-gluconate | Xanthomonas campestris pv. campestris | - |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + D-gluconate | Xanthomonas campestris pv. campestris ATCC 33913 | - |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | Q7S0S2 | - |
- |
Neurospora crassa DSM 1257 | Q7S0S2 | - |
- |
Xanthomonas campestris | Q8P3J4 | - |
- |
Xanthomonas campestris pv. campestris | Q8P3J4 | - |
- |
Xanthomonas campestris pv. campestris ATCC 33913 | Q8P3J4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Xanthomonas campestris pv. campestris |
- |
Neurospora crassa |
- |
Xanthomonas campestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | - |
Neurospora crassa | alpha-D-glucose 1 phosphate + D-gluconate | - |
r | |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | - |
Neurospora crassa DSM 1257 | alpha-D-glucose 1 phosphate + D-gluconate | - |
r | |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | - |
Xanthomonas campestris pv. campestris | alpha-D-glucose 1-phosphate + D-gluconate | - |
r | |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively | Neurospora crassa | alpha-D-glucose 1-phosphate + D-gluconate | - |
r | |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively | Xanthomonas campestris | alpha-D-glucose 1-phosphate + D-gluconate | - |
r | |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | 4-O-beta-D-glucopyranosyl-D-gluconate i.e. cellobionate. Phosphorolytic reaction follows a sequential bibi mechanism | Neurospora crassa | alpha-D-glucose 1-phosphate + D-gluconate | - |
r | |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | 4-O-beta-D-glucopyranosyl-D-gluconate i.e. cellobionate. Phosphorolytic reaction follows a sequential bibi mechanism | Xanthomonas campestris | alpha-D-glucose 1-phosphate + D-gluconate | - |
r | |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively | Neurospora crassa DSM 1257 | alpha-D-glucose 1-phosphate + D-gluconate | - |
r | |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | 4-O-beta-D-glucopyranosyl-D-gluconate i.e. cellobionate. Phosphorolytic reaction follows a sequential bibi mechanism | Neurospora crassa DSM 1257 | alpha-D-glucose 1-phosphate + D-gluconate | - |
r | |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | - |
Xanthomonas campestris pv. campestris ATCC 33913 | alpha-D-glucose 1-phosphate + D-gluconate | - |
r | |
alpha-D-glucose 1 phosphate + D-gluconate | - |
Neurospora crassa | 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | - |
r | |
alpha-D-glucose 1 phosphate + D-gluconate | - |
Neurospora crassa DSM 1257 | 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + D-gluconate | - |
Xanthomonas campestris pv. campestris | 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + D-gluconate | - |
Neurospora crassa | 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + D-gluconate | - |
Xanthomonas campestris | 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + D-gluconate | - |
Neurospora crassa DSM 1257 | 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + D-gluconate | - |
Xanthomonas campestris pv. campestris ATCC 33913 | 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + D-glucuronate | - |
Xanthomonas campestris pv. campestris | 4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + D-glucuronate | in the synthetic direction the enzyme produces 4-O-beta-D-glucopyranosyl-D-glucuronate from alpha-D-glucose 1-phosphate and D-glucuronate with low activity | Neurospora crassa | 4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate | - |
? | |
alpha-D-glucose 1-phosphate + D-glucuronate | in the synthetic direction the enzyme produces 4-O-beta-D-glucopyranosyl-D-glucuronate from alpha-D-glucose 1-phosphate and D-glucuronate with low activity | Xanthomonas campestris | 4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate | - |
? | |
alpha-D-glucose 1-phosphate + D-glucuronate | - |
Xanthomonas campestris pv. campestris ATCC 33913 | 4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 84000, SDS-PAGE | Xanthomonas campestris |
homodimer | 2 * 87000, SDS-PAGE | Neurospora crassa |
homodimer | 2 * 84000, SDS-PAGE, 2 * 89700, amino acid sequence | Xanthomonas campestris pv. campestris |
homodimer | 2 * 87000, SDS-PAGE, 2 * 90796, amino acid sequence | Neurospora crassa |
homodimer | 2 * 89700, calculated from sequence | Xanthomonas campestris |
homodimer | 2 * 90769, calculated from sequence | Neurospora crassa |
Synonyms | Comment | Organism |
---|---|---|
4-O-beta-D-glucopyranosyl-D-gluconic acid: phosphate alpha-D-glucosyltransferase | - |
Neurospora crassa |
4-O-beta-D-glucopyranosyl-D-gluconic acid: phosphate alpha-D-glucosyltransferase | - |
Xanthomonas campestris |
NCU09425 | locus name | Neurospora crassa |
XCC4077 | locus name | Xanthomonas campestris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Neurospora crassa |
30 | - |
assay at | Xanthomonas campestris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
15 min, stable | Neurospora crassa |
35 | - |
15 min, stable | Xanthomonas campestris |
35 | - |
15 min stable up to | Xanthomonas campestris pv. campestris |
35 | - |
15 min stable up to | Neurospora crassa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
43 | - |
D-glucuronate | pH 6.5, 30°C | Xanthomonas campestris | |
43 | - |
D-glucuronate | pH 6.0-6.5, 30°C | Xanthomonas campestris pv. campestris | |
127 | - |
alpha-D-glucose 1-phosphate | pH 6.5, 30°C | Xanthomonas campestris | |
127 | - |
alpha-D-glucose 1-phosphate | pH 6.0-6.5, 30°C | Xanthomonas campestris pv. campestris | |
146 | - |
D-gluconate | pH 6.5, 30°C | Neurospora crassa | |
146 | - |
D-gluconate | pH 6.0-6.5, 30°C | Neurospora crassa | |
173 | - |
D-gluconate | pH 6.5, 30°C | Xanthomonas campestris | |
173 | - |
D-gluconate | pH 6.0-6.5, 30°C | Xanthomonas campestris pv. campestris | |
306 | - |
phosphate | pH 7.0, 30°C | Neurospora crassa | |
306 | - |
4-O-beta-D-glucopyranosyl-D-gluconate | pH 7.0, 30°C | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
synthetic activity | Xanthomonas campestris |
6 | - |
synthetic reaction | Xanthomonas campestris pv. campestris |
6.5 | - |
synthetic activity | Neurospora crassa |
6.5 | - |
synthetic reaction | Neurospora crassa |
7 | - |
phosphorolytic reaction | Xanthomonas campestris pv. campestris |
7 | - |
phosphorolytic reaction | Neurospora crassa |
7 | - |
phosphorolytic activity | Neurospora crassa |
7 | - |
phosphorolytic activity | Xanthomonas campestris |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | 4°C, 24 h, stable | Xanthomonas campestris |
5 | 10.5 | - |
Xanthomonas campestris pv. campestris |
5.5 | 9 | - |
Neurospora crassa |
5.5 | 9 | 4°C, 24 h, stable | Neurospora crassa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
7.3 | - |
4-O-beta-D-glucopyranosyl-D-gluconate | pH 7.0, 30°C | Neurospora crassa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18 | - |
D-glucuronate | pH 6.5, 30°C | Xanthomonas campestris | |
87 | - |
D-gluconate | pH 6.5, 30°C | Xanthomonas campestris | |
605 | - |
alpha-D-glucose 1-phosphate | pH 6.5, 30°C | Xanthomonas campestris |