Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 | Ruminococcus albus |
expression in Escherichia coli BL21 (DE3) | Ruminococcus albus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.21 | - |
[(1->4)-beta-D-mannosyl]4 | pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction | Ruminococcus albus | |
3.21 | - |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man | pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate | Ruminococcus albus | |
4.48 | - |
[(1->4)-beta-D-mannosyl]6 | pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction | Ruminococcus albus | |
4.48 | - |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man | pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
4.55 | - |
[(1->4)-beta-D-mannosyl]5 | pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction | Ruminococcus albus | |
4.55 | - |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man | pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate | Ruminococcus albus | |
5.29 | - |
[(1->4)-beta-D-mannosyl]5 | pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction | Ruminococcus albus | |
5.29 | - |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man | pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
7.59 | - |
[(1->4)-beta-D-mannosyl]4 | pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction | Ruminococcus albus | |
7.59 | - |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man | pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
7.94 | - |
[(1->4)-beta-D-mannosyl]3 | pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction | Ruminococcus albus | |
7.94 | - |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man | pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate | Ruminococcus albus | |
13.3 | - |
[(1->4)-beta-D-mannosyl]3 | pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction | Ruminococcus albus | |
13.3 | - |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man | pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
28.4 | - |
4-O-beta-D-mannosyl-D-glucose | pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction | Ruminococcus albus | |
30.7 | - |
[(1->4)-beta-D-mannosyl]2 | pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction | Ruminococcus albus | |
30.7 | - |
beta-D-mannopyranosyl-(1->4)-D-mannopyranose | pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
44.5 | - |
[(1->4)-beta-D-mannosyl]2 | pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction | Ruminococcus albus | |
44.5 | - |
beta-D-mannopyranosyl-(1->4)-D-mannopyranose | pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate | Ruminococcus albus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Ruminococcus albus | 5737 | - |
intracellular | - |
Ruminococcus albus | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37823 | - |
6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence | Ruminococcus albus |
38000 | - |
6 * 38000, SDS-PAGE | Ruminococcus albus |
38000 | - |
6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence | Ruminococcus albus |
209000 | - |
gel filtration | Ruminococcus albus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[(1->4)-beta-D-mannosyl]n + phosphate | Ruminococcus albus | - |
[(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate | - |
r | |
[(1->4)-beta-D-mannosyl]n + phosphate | Ruminococcus albus | the enzyme is involved in the metabolic pathway of mannan | [(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ruminococcus albus | E6UBR9 | - |
- |
Ruminococcus albus NE1 | E6UBR9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Ruminococcus albus |
recombinant enzyme | Ruminococcus albus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[(1->4)-beta-D-mannosyl]n + phosphate = [(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate | n>=3, sequential bi-bi mechanism | Ruminococcus albus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.03 | - |
pH 6.5, 37°C | Ruminococcus albus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-mannosyl-D-glucose + phosphate | - |
Ruminococcus albus | D-glucose + alpha-D-mannosyl 1-phosphate | - |
? | |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate | - |
r | |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate | - |
r | |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate | - |
Ruminococcus albus | beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate | - |
r | |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate | best substrate for the synthetic reaction | Ruminococcus albus | beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate | - |
r | |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate | - |
Ruminococcus albus | beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate | - |
r | |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate | - |
r | |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate | - |
Ruminococcus albus | beta-D-mannopyranosyl-(1->4)-D-mannopyranose + alpha-D-mannose 1-phosphate | - |
r | |
beta-D-mannopyranosyl-(1->4)-beta-D-mannopyranosyl-(1->4)-D-mannopyranose + phosphate | - |
Ruminococcus albus | beta-D-mannopyranosyl-(1->4)-D-mannopyranose + alpha-D-mannose 1-phosphate | - |
r | |
beta-D-mannopyranosyl-(1->4)-D-mannopyranose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | beta-D-mannopyranosyl-(1->4)-beta-D-mannopyranosyl-(1->4)-D-mannopyranose + phosphate | - |
r | |
beta-D-mannopyranosyl-(1->4)-D-mannopyranose + phosphate | - |
Ruminococcus albus | D-mannose + alpha-D-mannose 1-phosphate | - |
r | |
additional information | The enzyme also catalyzes the reaction of EC 2.4.1.281, 4-O-beta-D-mannosyl-D-glucose phosphorylase | Ruminococcus albus | ? | - |
? | |
[(1->4)-beta-D-mannosyl]2 + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | [(1->4)-beta-D-mannosyl]3 + phosphate | - |
? | |
[(1->4)-beta-D-mannosyl]2 + phosphate | - |
Ruminococcus albus | D-mannose + alpha-D-mannose 1-phosphate | - |
? | |
[(1->4)-beta-D-mannosyl]3 + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | [(1->4)-beta-D-mannosyl]4 + phosphate | - |
? | |
[(1->4)-beta-D-mannosyl]3 + phosphate | - |
Ruminococcus albus | [(1->4)-beta-D-mannosyl]2 + alpha-D-mannose 1-phosphate | - |
? | |
[(1->4)-beta-D-mannosyl]4 + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | [(1->4)-beta-D-mannosyl]5 + phosphate | - |
? | |
[(1->4)-beta-D-mannosyl]4 + phosphate | - |
Ruminococcus albus | [(1->4)-beta-D-mannosyl]3 + alpha-D-mannose 1-phosphate | - |
? | |
[(1->4)-beta-D-mannosyl]5 + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | [(1->4)-beta-D-mannosyl]6 + phosphate | - |
? | |
[(1->4)-beta-D-mannosyl]5 + phosphate | - |
Ruminococcus albus | [(1->4)-beta-D-mannosyl]4 + alpha-D-mannose 1-phosphate | - |
? | |
[(1->4)-beta-D-mannosyl]6 + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | [(1->4)-beta-D-mannosyl]7 + phosphate | - |
? | |
[(1->4)-beta-D-mannosyl]6 + phosphate | - |
Ruminococcus albus | [(1->4)-beta-D-mannosyl]5 + alpha-D-mannose 1-phosphate | - |
? | |
[(1->4)-beta-D-mannosyl]n + phosphate | - |
Ruminococcus albus | [(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate | - |
r | |
[(1->4)-beta-D-mannosyl]n + phosphate | the enzyme is involved in the metabolic pathway of mannan | Ruminococcus albus | [(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate | - |
r | |
[(1->4)-beta-D-mannosyl]n + phosphate | the enzyme catalyses the reversible phosphorolysis of beta-1,4-mannooligosaccharide with a minimum size of three monomers. It acts on D-glucose derivatives at the C2- and C3-positions, including deoxy- and deoxyfluoro-analogues and epimers, but not on those substituted at the C6-position. It shows high synthetic activity toward the following oligosaccharides: beta-linked glucobioses, maltose, N,N'-diacetylchitobiose, and beta-1,4-mannooligosaccharides. Particularly, beta-1,4-mannooligosaccharides serve as significantly better acceptor substrates for the enzyme than D-glucose. In the phosphorolytic reactions, RaMP2 has weak activity toward beta-1,4-mannobiose but efficiently degrades beta-1,4-mannooligosaccharides longer than beta-1,4-mannobiose. It is thought to catalyze the phosphorolysis of beta-1,4-mannooligosaccharides longer than beta-1,4-mannobiose to produce alpha-D-mannose 1-phosphate and beta-1,4-mannobiose | Ruminococcus albus | [(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate | - |
r | |
[(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate | the enzyme catalyses the reversible phosphorolysis of beta-1,4-mannooligosaccharide with a minimum size of three monomers. It acts on D-glucose derivatives at the C2- and C3-positions, including deoxy- and deoxyfluoro-analogues and epimers, but not on those substituted at the C6-position. It shows high synthetic activity toward the following oligosaccharides: beta-linked glucobioses, maltose, N,N'-diacetylchitobiose, and beta-1,4-mannooligosaccharides. Particularly, beta-1,4-mannooligosaccharides serve as significantly better acceptor substrates for the enzyme than D-glucose. In the phosphorolytic reactions, RaMP2 has weak activity toward beta-1,4-mannobiose but efficiently degrades beta-1,4-mannooligosaccharides longer than beta-1,4-mannobiose. It is thought to catalyze the phosphorolysis of beta-1,4-mannooligosaccharides longer than beta-1,4-mannobiose to produce alpha-D-mannose 1-phosphate and beta-1,4-mannobiose | Ruminococcus albus | [(1->4)-beta-D-mannosyl]n + phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 38000, SDS-PAGE | Ruminococcus albus |
homohexamer | 6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence | Ruminococcus albus |
Synonyms | Comment | Organism |
---|---|---|
RaMp2 | - |
Ruminococcus albus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Ruminococcus albus |
45 | - |
- |
Ruminococcus albus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to | Ruminococcus albus |
40 | - |
stable below | Ruminococcus albus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.06 | - |
[(1->4)-beta-D-mannosyl]2 | pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction | Ruminococcus albus | |
7.06 | - |
beta-D-mannopyranosyl-(1->4)-D-mannopyranose | pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate | Ruminococcus albus | |
16 | - |
[(1->4)-beta-D-mannosyl]6 | pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction | Ruminococcus albus | |
16 | - |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man | pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
20.5 | - |
[(1->4)-beta-D-mannosyl]5 | pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction | Ruminococcus albus | |
20.5 | - |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man | pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
20.7 | - |
4-O-beta-D-mannosyl-D-glucose | pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction | Ruminococcus albus | |
27.5 | - |
[(1->4)-beta-D-mannosyl]3 | pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction | Ruminococcus albus | |
27.5 | - |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man | pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate | Ruminococcus albus | |
31.2 | - |
[(1->4)-beta-D-mannosyl]4 | pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction | Ruminococcus albus | |
31.2 | - |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man | pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
31.9 | - |
[(1->4)-beta-D-mannosyl]5 | pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction | Ruminococcus albus | |
31.9 | - |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man | pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate | Ruminococcus albus | |
33.1 | - |
[(1->4)-beta-D-mannosyl]4 | pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction | Ruminococcus albus | |
33.1 | - |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man | pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate | Ruminococcus albus | |
34.6 | - |
[(1->4)-beta-D-mannosyl]3 | pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction | Ruminococcus albus | |
34.6 | - |
beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man | pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
45.1 | - |
[(1->4)-beta-D-mannosyl]2 | pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction | Ruminococcus albus | |
45.1 | - |
beta-D-mannopyranosyl-(1->4)-D-mannopyranose | pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Ruminococcus albus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 9.5 | stable | Ruminococcus albus |
3.5 | 9.5 | 4°C, 24 h stable | Ruminococcus albus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the metabolic pathway of mannan | Ruminococcus albus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.159 | - |
[(1->4)-beta-D-mannosyl]2 | pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction | Ruminococcus albus | |
0.729 | - |
4-O-beta-D-mannosyl-D-glucose | pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction | Ruminococcus albus | |
1.47 | - |
[(1->4)-beta-D-mannosyl]2 | pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction | Ruminococcus albus | |
2.6 | - |
[(1->4)-beta-D-mannosyl]3 | pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction | Ruminococcus albus | |
3.46 | - |
[(1->4)-beta-D-mannosyl]3 | pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction | Ruminococcus albus | |
3.57 | - |
[(1->4)-beta-D-mannosyl]6 | pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction | Ruminococcus albus | |
3.87 | - |
[(1->4)-beta-D-mannosyl]5 | pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction | Ruminococcus albus | |
4.11 | - |
[(1->4)-beta-D-mannosyl]4 | pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction | Ruminococcus albus | |
7.01 | - |
[(1->4)-beta-D-mannosyl]5 | pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction | Ruminococcus albus | |
10.3 | - |
[(1->4)-beta-D-mannosyl]4 | pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction | Ruminococcus albus |