Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 8.2% residual activity at 5 mM | Panax notoginseng | |
Cu2+ | 2.2% residual activity at 5 mM | Panax notoginseng | |
Fe2+ | 2.3% residual activity at 5 mM | Panax notoginseng | |
Hg2+ | complete inhibition at 5 mM | Panax notoginseng | |
additional information | the activity of the enzyme is not apparently affected by EDTA | Panax notoginseng | |
Zn2+ | complete inhibition at 5 mM | Panax notoginseng |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
ginsenoside Rd | in 50 mM Tris-HCl buffer, at pH 8.5 and 35°C | Panax notoginseng | |
0.32 | - |
UDP-alpha-D-glucose | in 50 mM Tris-HCl buffer, at pH 8.5 and 35°C | Panax notoginseng |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 129% activity at 5 mM | Panax notoginseng | |
Mg2+ | 111.9% activity at 5 mM | Panax notoginseng | |
Mn2+ | 133.2% activity at 5 mM | Panax notoginseng | |
additional information | the activity of the enzyme is not apparently affected by Na+ or K+ | Panax notoginseng |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
x * 36000, SDS-PAGE | Panax notoginseng |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + ginsenoside Rd | Panax notoginseng | over 80% yield after 10 h | UDP + ginsenoside Rb1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Panax notoginseng | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, HiTrap Q anion-exchange chromatography, and Sephadex G-25 gel filtration | Panax notoginseng |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0003 | - |
crude extract, at pH 8.5 and 35°C | Panax notoginseng |
0.044 | - |
after 145.8fold purification, at pH 8.5 and 35°C | Panax notoginseng |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + ginsenoside Rd | over 80% yield after 10 h | Panax notoginseng | UDP + ginsenoside Rb1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, SDS-PAGE | Panax notoginseng |
Synonyms | Comment | Organism |
---|---|---|
UDPG:ginsenoside Rd glucosyltransferase | - |
Panax notoginseng |
UGRdGT | - |
Panax notoginseng |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Panax notoginseng |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Panax notoginseng |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | more than 60% activity between pH 7.0 and 9.0 | Panax notoginseng |