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Literature summary for 2.4.1.314 extracted from

  • Yue, C.; Zhong, J.
    Purification and characterization of UDPG:ginsenoside Rd glucosyltransferase from suspended cells of Panax notoginseng (2005), Process Biochem., 40, 3742-3748.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 8.2% residual activity at 5 mM Panax notoginseng
Cu2+ 2.2% residual activity at 5 mM Panax notoginseng
Fe2+ 2.3% residual activity at 5 mM Panax notoginseng
Hg2+ complete inhibition at 5 mM Panax notoginseng
additional information the activity of the enzyme is not apparently affected by EDTA Panax notoginseng
Zn2+ complete inhibition at 5 mM Panax notoginseng

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
ginsenoside Rd in 50 mM Tris-HCl buffer, at pH 8.5 and 35°C Panax notoginseng
0.32
-
UDP-alpha-D-glucose in 50 mM Tris-HCl buffer, at pH 8.5 and 35°C Panax notoginseng

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 129% activity at 5 mM Panax notoginseng
Mg2+ 111.9% activity at 5 mM Panax notoginseng
Mn2+ 133.2% activity at 5 mM Panax notoginseng
additional information the activity of the enzyme is not apparently affected by Na+ or K+ Panax notoginseng

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
x * 36000, SDS-PAGE Panax notoginseng

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + ginsenoside Rd Panax notoginseng over 80% yield after 10 h UDP + ginsenoside Rb1
-
?

Organism

Organism UniProt Comment Textmining
Panax notoginseng
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, HiTrap Q anion-exchange chromatography, and Sephadex G-25 gel filtration Panax notoginseng

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0003
-
crude extract, at pH 8.5 and 35°C Panax notoginseng
0.044
-
after 145.8fold purification, at pH 8.5 and 35°C Panax notoginseng

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + ginsenoside Rd over 80% yield after 10 h Panax notoginseng UDP + ginsenoside Rb1
-
?

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE Panax notoginseng

Synonyms

Synonyms Comment Organism
UDPG:ginsenoside Rd glucosyltransferase
-
Panax notoginseng
UGRdGT
-
Panax notoginseng

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Panax notoginseng

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Panax notoginseng

pH Range

pH Minimum pH Maximum Comment Organism
7 9 more than 60% activity between pH 7.0 and 9.0 Panax notoginseng