Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a hexahistidine-tagged protein | Amycolatopsis orientalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
vancomycin aglycone | severe substrate inhibition above 0.6 mM | Amycolatopsis orientalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
vancomycin aglycone | pH 9, 37°C | Amycolatopsis orientalis | |
1.3 | - |
UDP-alpha-D-glucose | pH 9, 37°C | Amycolatopsis orientalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 44000, C-terminal His6-tagged version of GtfB, SDS-PAGE | Amycolatopsis orientalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + vancomycin aglycone | Amycolatopsis orientalis | the enzyme is involved in the biosynthesis of chloroeremomycin | UDP + devancoaminyl vancomycin | - |
? | |
UDP-alpha-D-glucose + vancomycin aglycone | Amycolatopsis orientalis A82846 | the enzyme is involved in the biosynthesis of chloroeremomycin | UDP + devancoaminyl vancomycin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amycolatopsis orientalis | P96559 | - |
- |
Amycolatopsis orientalis A82846 | P96559 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Amycolatopsis orientalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + teicoplanin aglycone | very low glucosylation activity | Amycolatopsis orientalis | UDP + ? | - |
? | |
UDP-alpha-D-glucose + teicoplanin aglycone | very low glucosylation activity | Amycolatopsis orientalis A82846 | UDP + ? | - |
? | |
UDP-alpha-D-glucose + vancomycin aglycone | - |
Amycolatopsis orientalis | UDP + devancoaminyl vancomycin | - |
? | |
UDP-alpha-D-glucose + vancomycin aglycone | the enzyme is involved in the biosynthesis of chloroeremomycin | Amycolatopsis orientalis | UDP + devancoaminyl vancomycin | - |
? | |
UDP-alpha-D-glucose + vancomycin aglycone | - |
Amycolatopsis orientalis A82846 | UDP + devancoaminyl vancomycin | - |
? | |
UDP-alpha-D-glucose + vancomycin aglycone | the enzyme is involved in the biosynthesis of chloroeremomycin | Amycolatopsis orientalis A82846 | UDP + devancoaminyl vancomycin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, C-terminal His6-tagged version of GtfB, SDS-PAGE | Amycolatopsis orientalis |
Synonyms | Comment | Organism |
---|---|---|
GtfB | - |
Amycolatopsis orientalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Amycolatopsis orientalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.283 | - |
UDP-alpha-D-glucose | pH 9, 37°C | Amycolatopsis orientalis | |
0.283 | - |
vancomycin aglycone | pH 9, 37°C | Amycolatopsis orientalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Amycolatopsis orientalis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of chloroeremomycin | Amycolatopsis orientalis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.88 | - |
vancomycin aglycone | pH 9, 37°C | Amycolatopsis orientalis | |
4.6 | - |
UDP-alpha-D-glucose | pH 9, 37°C | Amycolatopsis orientalis |