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Literature summary for 2.4.1.310 extracted from

  • Losey, H.C.; Peczuh, M.W.; Chen, Z.; Eggert, U.S.; Dong, S.D.; Pelczer, I.; Kahne, D.; Walsh, C.T.
    Tandem action of glycosyltransferases in the maturation of vancomycin and teicoplanin aglycones: novel glycopeptides (2001), Biochemistry, 40, 4745-4755.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as a hexahistidine-tagged protein Amycolatopsis orientalis

Inhibitors

Inhibitors Comment Organism Structure
vancomycin aglycone severe substrate inhibition above 0.6 mM Amycolatopsis orientalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
vancomycin aglycone pH 9, 37°C Amycolatopsis orientalis
1.3
-
UDP-alpha-D-glucose pH 9, 37°C Amycolatopsis orientalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
x * 44000, C-terminal His6-tagged version of GtfB, SDS-PAGE Amycolatopsis orientalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + vancomycin aglycone Amycolatopsis orientalis the enzyme is involved in the biosynthesis of chloroeremomycin UDP + devancoaminyl vancomycin
-
?
UDP-alpha-D-glucose + vancomycin aglycone Amycolatopsis orientalis A82846 the enzyme is involved in the biosynthesis of chloroeremomycin UDP + devancoaminyl vancomycin
-
?

Organism

Organism UniProt Comment Textmining
Amycolatopsis orientalis P96559
-
-
Amycolatopsis orientalis A82846 P96559
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Amycolatopsis orientalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + teicoplanin aglycone very low glucosylation activity Amycolatopsis orientalis UDP + ?
-
?
UDP-alpha-D-glucose + teicoplanin aglycone very low glucosylation activity Amycolatopsis orientalis A82846 UDP + ?
-
?
UDP-alpha-D-glucose + vancomycin aglycone
-
Amycolatopsis orientalis UDP + devancoaminyl vancomycin
-
?
UDP-alpha-D-glucose + vancomycin aglycone the enzyme is involved in the biosynthesis of chloroeremomycin Amycolatopsis orientalis UDP + devancoaminyl vancomycin
-
?
UDP-alpha-D-glucose + vancomycin aglycone
-
Amycolatopsis orientalis A82846 UDP + devancoaminyl vancomycin
-
?
UDP-alpha-D-glucose + vancomycin aglycone the enzyme is involved in the biosynthesis of chloroeremomycin Amycolatopsis orientalis A82846 UDP + devancoaminyl vancomycin
-
?

Subunits

Subunits Comment Organism
? x * 44000, C-terminal His6-tagged version of GtfB, SDS-PAGE Amycolatopsis orientalis

Synonyms

Synonyms Comment Organism
GtfB
-
Amycolatopsis orientalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Amycolatopsis orientalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.283
-
UDP-alpha-D-glucose pH 9, 37°C Amycolatopsis orientalis
0.283
-
vancomycin aglycone pH 9, 37°C Amycolatopsis orientalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Amycolatopsis orientalis

General Information

General Information Comment Organism
physiological function the enzyme is involved in the biosynthesis of chloroeremomycin Amycolatopsis orientalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.88
-
vancomycin aglycone pH 9, 37°C Amycolatopsis orientalis
4.6
-
UDP-alpha-D-glucose pH 9, 37°C Amycolatopsis orientalis