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Literature summary for 2.4.1.31 extracted from

  • Kuhaudomlarp, S.; Stevenson, C.E.M.; Lawson, D.M.; Field, R.A.
    The structure of a GH149 beta-(1->3) glucan phosphorylase reveals a new surface oligosaccharide binding site and additional domains that are absent in the disaccharide-specific GH94 glucose-beta-(1->3)-glucose (laminaribiose) phosphorylase (2019), Proteins, 87, 885-892 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells uncultured bacterium

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in the absence of substrate and in complex with laminarihexaose, sitting drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.05 M magnesium sulfate heptahydrate, 0.1 M bicine pH 9.0, and 20% (v/v) polyethylene glycol uncultured bacterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-D-glucose 1-phosphate + D-glucose uncultured bacterium
-
 laminaribiose + phosphate
-
 r

Organism

Organism UniProt Comment Textmining
uncultured bacterium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex S200 gel filtration uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate + D-glucose
-
 uncultured bacterium laminaribiose + phosphate
-
 r

Synonyms

Synonyms Comment Organism
glucose-beta-(1->3)-glucose phosphorylase
-
 uncultured bacterium
Pro_7066
-
 uncultured bacterium