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Literature summary for 2.4.1.303 extracted from

  • Riley, J.G.; Menggad, M.; Montoya-Peleaz, P.J.; Szarek, W.A.; Marolda, C.L.; Valvano, M.A.; Schutzbach, J.S.; Brockhausen, I.
    The wbbD gene of E. coli strain VW187 (O7:K1) encodes a UDP-Gal: GlcNAcalpha-pyrophosphate-R beta1,3-galactosyltransferase involved in the biosynthesis of O7-specific lipopolysaccharide (2005), Glycobiology, 15, 605-613.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
additional information detergents in the assay do not increase glycosyl transfer Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a GST-fusion protein encoded by pCM227 in DH5alpha Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
CHAPS activity is diminished in the presence of CHAPS at concentrations of 0.125–0.6% Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 1-O-(11-phenoxyundecyl-diphosphoryl)-alpha-D-glucopyranoside pH 7.0, 37°C Escherichia coli
1.2
-
 UDP-alpha-D-galactose pH 7.0, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 5 mM, yields 38% activity, respectively, compared with 5 mM MnCl2 Escherichia coli
Mg2+ 5 mM, yields 11% activity, respectively, compared with 5 mM MnCl2 Escherichia coli
Mn2+ optimal activity in presence of 2.5 mM Mn2+. Without addition of divalent metal the activity is less than 4.5% of the activity in presence of Mn2+ Escherichia coli
Zn2+ 5 mM, yields 9% activity, respectively, compared with 5 mM MnCl2 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli VW187
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-galactose + 1-O-(11-phenoxyundecyl-diphosphoryl)-alpha-D-glucopyranoside synthetic substrate, which is an analog of N-acetyl-D-glucosaminyldiphospho-ditrans,octacis-undecaprenol Escherichia coli UDP + 1-O-(11-phenoxyundecyl-diphosphoryl)-3-O-beta-D-galactopyranosyl-alpha-D-glucopyranoside
-
 ?
UDP-alpha-D-galactose + 1-O-(11-phenoxyundecyl-diphosphoryl)-alpha-D-glucopyranoside synthetic substrate, which is an analog of N-acetyl-D-glucosaminyldiphospho-ditrans,octacis-undecaprenol Escherichia coli VW187 UDP + 1-O-(11-phenoxyundecyl-diphosphoryl)-3-O-beta-D-galactopyranosyl-alpha-D-glucopyranoside
-
 ?

Synonyms

Synonyms Comment Organism
UDP-Gal:GlcNAcalpha-pyrophosphate-R beta1,3-galactosyltransferase
-
 Escherichia coli
WbbD
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8 broad optimum Escherichia coli

General Information

General Information Comment Organism
malfunction a mutant strain with a defective wbbD gene is unable to form O7 LPS and lacks this specific galactosyltransferase activity. The normal phenotype is restored by complementing the mutant with the cloned wbbD gene Escherichia coli
physiological function the enzyme is involved in the biosynthesis of the O7-specific lipopolysaccharide Escherichia coli