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Literature summary for 2.4.1.30 extracted from
- beta-1,3-Oligoglucan:orthophosphate glucosyltransferases from Euglena gracilis. I. Isolation and some properties of a beta-1,3-oligoglucan phosphorylase (1967), Biochim. Biophys. Acta, 146, 417-430.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | absolute requirement for sulfhydryl groups | Euglena gracilis |
General Stability
| General Stability | Organism |
|---|---|
| without 2-mercaptoethanol: loss of 80% activity after 2 weeks and over 95% after 8 weeks in enzyme extracts, with 2-mercaptoethanol: loss of 50% activity after 8 weeks | Euglena gracilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroquinone | strongly inhibits in absence of 2-mercaptoethanol | Euglena gracilis |  |
| UMP | in phosphorolysis, 30% inhibition at 3 mM | Euglena gracilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
alpha-D-glucose 1-phosphate | + laminaribiose | Euglena gracilis | |
| 2 | - |
Laminaripentaose | - |
Euglena gracilis | |
| 2 | - |
phosphate | pH 7.2, with laminaribiose | Euglena gracilis | |
| 3 | - |
laminaritetraose | - |
Euglena gracilis | |
| 3 | - |
phosphate | pH 7.2, with laminaritetraose | Euglena gracilis | |
| 4 | - |
laminaribiose | - |
Euglena gracilis | |
| 4.5 | - |
laminaritriose | - |
Euglena gracilis | |
| 40 | - |
glucose | - |
Euglena gracilis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | Euglena gracilis | - |
(1,3-beta-D-glucosyl)n + phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euglena gracilis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.3 | - |
laminaribiose | Euglena gracilis |
| 0.5 | - |
glucose | Euglena gracilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
Euglena gracilis | (1,3-beta-D-glucosyl)n + phosphate | - |
r | |
| arbutin + alpha-D-glucose 1-phosphate | - |
Euglena gracilis | 1,3-beta-D-glucosylarbutin + phosphate | - |
r | |
| beta-methylglucoside + alpha-D-glucose 1-phosphate | - |
Euglena gracilis | 1,3-beta-D-glucosyl-beta-methylglucoside + phosphate | - |
r | |
| beta-phenylglucoside + alpha-D-glucose 1-phosphate | - |
Euglena gracilis | 1,3-beta-D-glucosyl-beta-phenylglucoside + phosphate | - |
r | |
| cellobiose + alpha-D-glucose 1-phosphate | - |
Euglena gracilis | ? | - |
r | |
| D-glucose + alpha-D-glucose 1-phosphate | - |
Euglena gracilis | laminaribiose + phosphate | - |
r | |
| gentiobiose + alpha-D-glucose 1-phosphate | - |
Euglena gracilis | 1,3-beta-D-glucosylgentiobiose + phosphate | - |
r | |
| laminaribiose + alpha-D-glucose 1-phosphate | - |
Euglena gracilis | laminaritriose + phosphate | product analysis method | r | |
| laminaribiosyl-beta-1,4-glucose + alpha-D-glucose 1-phosphate | - |
Euglena gracilis | laminaritriosyl-beta-1,4-glucose + phosphate | - |
r | |
| laminaribiosyl-p-hydroquinone + alpha-D-glucose 1-phosphate | - |
Euglena gracilis | laminaritriosyl-p-hydroquinone + phosphate | - |
r | |
| laminaribiosyl-saligenin + alpha-D-glucose 1-phosphate | - |
Euglena gracilis | laminaritriosyl-saligenin + phosphate | - |
r | |
| laminaridextrins + alpha-D-glucose 1-phosphate | - |
Euglena gracilis | ? | - |
r | |
| laminariheptaose + alpha-D-glucose 1-phosphate | - |
Euglena gracilis | laminarioctaose + phosphate | - |
r | |
| laminaripentaose + alpha-D-glucose 1-phosphate | low activity | Euglena gracilis | laminarihexaose + phosphate | - |
r | |
| laminaritetraose + alpha-D-glucose 1-phosphate | low activity | Euglena gracilis | laminaripentaose + phosphate | - |
r | |
| laminaritetraosyl-saligenin + alpha-D-glucose 1-phosphate | - |
Euglena gracilis | laminaripentaosyl-saligenin + phosphate | - |
r | |
| laminaritriose + alpha-D-glucose 1-phosphate | - |
Euglena gracilis | laminaritetraose + phosphate | - |
r | |
| laminaritriosyl-p-hydroquinone + alpha-D-glucose 1-phosphate | - |
Euglena gracilis | laminaritetraosyl-p-hydroquinone + phosphate | - |
r | |
| laminaritriosyl-saligenin + alpha-D-glucose 1-phosphate | - |
Euglena gracilis | laminaritetraosyl-saligenin + phosphate | - |
r | |
| additional information | does not act on laminarin and paramylon | Euglena gracilis | ? | - |
? | |
| salicin + alpha-D-glucose 1-phosphate | - |
Euglena gracilis | 1,3-beta-D-glucosylsalicin + phosphate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | not identical with laminaribiose phosphorylase, which catalyzes the same reaction | Euglena gracilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 37 | assay at | Euglena gracilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | glucose or laminaribiose as acceptor | Euglena gracilis |
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Release 2023.1 (January 2023)
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