Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by EDTA at 5 mM | Petunia x hybrida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
delphinidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] | pH 8.3, 30°C | Petunia x hybrida | |
0.003 | - |
petunidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] | pH 8.3, 30°C | Petunia x hybrida | |
0.22 | - |
UDP-alpha-D-glucose | pH 8.3, 30°C | Petunia x hybrida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no stimulation by 1 mM Mg2+ or Ca2+ | Petunia x hybrida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 52000, SDS-PAGE | Petunia x hybrida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + delphinidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] | Petunia x hybrida | i.e. delphinidin 3-(4-coumaroyl)-rutinoside | UDP + delphinidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] 5-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + petunidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] | Petunia x hybrida | i.e. petunidin 3-(4-coumaroyl)-rutinoside | UDP + petunidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] 5-O-beta-D-glucoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petunia x hybrida | - |
- |
- |
Petunia x hybrida | - |
gene gene Anl | - |
Purification (Comment) | Organism |
---|---|
30fold from flowers, by ammonium sulfate fractionation, desalting gel filtration, separation of the 5-O-glucosyltransferase from the anthocyanidin 3-O-glucosyltransferase by chromatofocusing, followed by ultrafiltration | Petunia x hybrida |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Petunia x hybrida | - |
pollen | - |
Petunia x hybrida | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.05 | - |
purified native enzyme, pH 8.3, 30°C | Petunia x hybrida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity, overview. No activity with delphinidin 3-rutinoside, cyanidin 3-rutinoside, and delphinidin 3-glucoside | Petunia x hybrida | ? | - |
? | |
UDP-alpha-D-glucose + delphinidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] | i.e. delphinidin 3-(4-coumaroyl)-rutinoside | Petunia x hybrida | UDP + delphinidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] 5-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + petunidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] | i.e. petunidin 3-(4-coumaroyl)-rutinoside | Petunia x hybrida | UDP + petunidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] 5-O-beta-D-glucoside | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, SDS-PAGE | Petunia x hybrida |
Synonyms | Comment | Organism |
---|---|---|
anthocyanin 5-O-glucosyltransferase | - |
Petunia x hybrida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Petunia x hybrida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Petunia x hybrida |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Petunia x hybrida | chromatofocusing | - |
4.75 |