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Literature summary for 2.4.1.294 extracted from

  • Feng, K.; Xu, Z.S.; Liu, J.X.; Li, J.W.; Wang, F.; Xiong, A.S.
    Isolation, purification, and characterization of AgUCGalT1, a galactosyltransferase involved in anthocyanin galactosylation in purple celery (Apium graveolens L.) (2018), Planta, 247, 1363-1375 .
    View publication on PubMed
Search for more literature for 2.4.1.294

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Apium graveolens
expression in Escherichia coli Apium graveolens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0092
-
 cyanidin pH 8.0, 30°C Apium graveolens
0.0092
-
 cyanidin pH 8.0, 35°C Apium graveolens

Organism

Organism UniProt Comment Textmining
Apium graveolens A0A346XR64
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Apium graveolens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no donors: UDP-glucose and UDP-xylose Apium graveolens ?
-
-
UDP-alpha-D-galactose + cyanidin
-
 Apium graveolens UDP + cyanidin 3-O-beta-D-galactoside
-
 ?
UDP-alpha-D-galactose + kaempferol
-
 Apium graveolens UDP + kaempferol 3-O-beta-D-galactoside
-
 ?
UDP-alpha-D-galactose + peonidin
-
 Apium graveolens UDP + peonidin 3-O-beta-D-galactoside
-
 ?
UDP-alpha-D-galactose + quercetin
-
 Apium graveolens UDP + quercetin 3-O-beta-D-galactoside
-
 ?
UDP-D-galactose + peonidin 26% of the activity compared to cyanidin Apium graveolens UDP + peonidin 3-O-beta-D-galactoside
-
 ?
UDP-D-galactose + quercetin 25% of the activity compared to cyanidin Apium graveolens UDP + quercetin 3-O-beta-D-galactoside
-
 ?
UDP-galactose + cyanidin
-
 Apium graveolens UDP + cyanidin 3-O-beta-D-galactoside
-
 ?
UDP-galactose + kaempferol reaction of EC 2.4.1.234, 53% of the activity compared to cyanidin Apium graveolens UDP + kaempferol 3-O-beta-D-galactoside
-
 ?

Subunits

Subunits Comment Organism
? x * 49570, calculated from sequence, x * 55000, SDS-PAGE Apium graveolens

Synonyms

Synonyms Comment Organism
DP-galactose:cyanidin 3-O-galactosyltransferase
-
 Apium graveolens
UCGalT1
-
 Apium graveolens
UDP-galactose:cyanidin 3-O-galactosyltransferase
-
 Apium graveolens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Apium graveolens
35
-
 activity with cyanidin and UDP-galactose Apium graveolens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 45 20°C: about 40% of maximal activity, 45°C: about 60% of maximal activity, activity with cyanidin and UDP-galactose Apium graveolens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.16
-
 cyanidin pH 8.0, 30°C Apium graveolens
6.16
-
 cyanidin pH 8.0, 35°C Apium graveolens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Apium graveolens
8
-
 activity with cyanidin and UDP-galactose Apium graveolens

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5 pH 7.0: about 40% of maximal activity, pH 8.5: about 20% of maximal activity, activity with cyanidin and UDP-galactose Apium graveolens

Expression

Organism Comment Expression
Apium graveolens expression levels of UCGalT1 are positively correlated with the total anthocyanin contents in purple and non-purple celery varieties additional information

General Information

General Information Comment Organism
metabolism the enzyme is involved in anthocyanin galactosylation in Apium graveolens Apium graveolens