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Literature summary for 2.4.1.288 extracted from
- Fidelity and promiscuity of a mycobacterial glycosyltransferase (2016), J. Am. Chem. Soc., 138, 9205-9211 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose | pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis |  |
| 0.013 | - |
D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 0.029 | - |
D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose | pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 0.034 | - |
D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 0.066 | - |
D-galactofuranosyl-(1->6)-beta-D-galactofuranose | pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 0.19 | - |
D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Mycobacterium tuberculosis | GlfT2 is promiscuous in its substrate preferences. It preferentially acts on substrates in which the lipid-bearing Gal residue is connected to the sequence by a (1->)-beta-glycosidic linkage. The preferential formation of alternating (1->6)-beta-Galf and (1->5)-beta-Galf residues arises not from substrate binding but during catalytic processive polymerization | ? | - |
- |
|
| additional information | Mycobacterium tuberculosis ATCC 25618 | GlfT2 is promiscuous in its substrate preferences. It preferentially acts on substrates in which the lipid-bearing Gal residue is connected to the sequence by a (1->)-beta-glycosidic linkage. The preferential formation of alternating (1->6)-beta-Galf and (1->5)-beta-Galf residues arises not from substrate binding but during catalytic processive polymerization | ? | - |
- |
|
| UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | Mycobacterium tuberculosis | - |
UDP + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | - |
? | |
| UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | Mycobacterium tuberculosis ATCC 25618 | - |
UDP + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | - |
? | |
| UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->6)-beta-D-galactofuranose | Mycobacterium tuberculosis | - |
UDP + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | - |
? | |
| UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->6)-beta-D-galactofuranose | Mycobacterium tuberculosis ATCC 25618 | - |
UDP + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | - |
? | |
| UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose | Mycobacterium tuberculosis | - |
UDP + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose | - |
? | |
| UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | Mycobacterium tuberculosis | natural substrate | UDP + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | O53585 | - |
- |
| Mycobacterium tuberculosis ATCC 25618 | O53585 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | GlfT2 is promiscuous in its substrate preferences. It preferentially acts on substrates in which the lipid-bearing Gal residue is connected to the sequence by a (1->)-beta-glycosidic linkage. The preferential formation of alternating (1->6)-beta-Galf and (1->5)-beta-Galf residues arises not from substrate binding but during catalytic processive polymerization | Mycobacterium tuberculosis | ? | - |
- |
|
| additional information | GlfT2 is promiscuous in its substrate preferences. It preferentially acts on substrates in which the lipid-bearing Gal residue is connected to the sequence by a (1->6)-beta-glycosidic linkage. The preferential formation of alternating (1->6)-beta-Galf and (1->5)-beta-Galf residues arises not from substrate binding but during catalytic processive polymerization | Mycobacterium tuberculosis | ? | - |
- |
|
| additional information | GlfT2 is promiscuous in its substrate preferences. It preferentially acts on substrates in which the lipid-bearing Gal residue is connected to the sequence by a (1->)-beta-glycosidic linkage. The preferential formation of alternating (1->6)-beta-Galf and (1->5)-beta-Galf residues arises not from substrate binding but during catalytic processive polymerization | Mycobacterium tuberculosis ATCC 25618 | ? | - |
- |
|
| additional information | GlfT2 is promiscuous in its substrate preferences. It preferentially acts on substrates in which the lipid-bearing Gal residue is connected to the sequence by a (1->6)-beta-glycosidic linkage. The preferential formation of alternating (1->6)-beta-Galf and (1->5)-beta-Galf residues arises not from substrate binding but during catalytic processive polymerization | Mycobacterium tuberculosis ATCC 25618 | ? | - |
- |
|
| UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose | nonnatural substrate | Mycobacterium tuberculosis | UDP + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose | - |
? | |
| UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | - |
Mycobacterium tuberculosis | UDP + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | - |
? | |
| UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | natural substrate | Mycobacterium tuberculosis | UDP + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | - |
? | |
| UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | - |
Mycobacterium tuberculosis ATCC 25618 | UDP + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | - |
? | |
| UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | natural substrate | Mycobacterium tuberculosis ATCC 25618 | UDP + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | - |
? | |
| UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->6)-beta-D-galactofuranose | - |
Mycobacterium tuberculosis | UDP + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | - |
? | |
| UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->6)-beta-D-galactofuranose | - |
Mycobacterium tuberculosis ATCC 25618 | UDP + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | - |
? | |
| UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose | - |
Mycobacterium tuberculosis | UDP + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose | - |
? | |
| UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose | natural substrate | Mycobacterium tuberculosis | UDP + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose | - |
? | |
| UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | natural substrate | Mycobacterium tuberculosis | UDP + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | - |
? | |
| UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | nonnatural substrate | Mycobacterium tuberculosis | UDP + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlfT2 | - |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.72 | - |
D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 0.87 | - |
D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose | pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 1 | - |
D-galactofuranosyl-(1->6)-beta-D-galactofuranose | pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 2.9 | - |
D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 3.7 | - |
D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose | pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 5.3 | - |
D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.8 | - |
D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 15 | - |
D-galactofuranosyl-(1->6)-beta-D-galactofuranose | pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 30 | - |
D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose | pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 160 | - |
D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 220 | - |
D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose | pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 280 | - |
D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose | pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
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