Literature summary for 2.4.1.283 extracted from

  • Yokoyama, K.; Yamamoto, Y.; Kudo, F.; Eguchi, T.
    Involvement of two distinct N-acetylglucosaminyltransferases and a dual-function deacetylase in neomycin biosynthesis (2008), ChemBioChem, 9, 865-869.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a His-tagged fusion protein in Escherichia coli Streptomyces fradiae
co-expressed with molecular chaperone GroES and GroEL in Escherichia coli BL21(DE3) cells Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces fradiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
a poor amount is isolated using Ni-NTA chromatography. The cell-free extract of Escherichia coli expressing NeoD is used for enzymatic assays Streptomyces fradiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information other possible glycosyl donor, UDP-glucose, and other possible glycosyl acceptors, paromamine, neamine, and ribostamycin are not accepted Streptomyces fradiae ?
-
?
additional information the enzyme is unable to catalyze the glycosylation of ribostamycin Streptomyces sp. ?
-
?
UDP-N-acetyl-alpha-D-glucosamine + 2-deoxystreptamine
-
Streptomyces fradiae UDP + 2'-N-acetylparomamine
-
?
UDP-N-acetyl-alpha-D-glucosamine + 2-deoxystreptamine about 80% of 2-deoxystreptamine is conversed Streptomyces sp. UDP + 2'-N-acetylparomamine
-
?

Synonyms

Synonyms Comment Organism
neoD
-
Streptomyces sp.
neoD
-
Streptomyces fradiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Streptomyces fradiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Streptomyces fradiae