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Literature summary for 2.4.1.281 extracted from

  • Kawahara, R.; Saburi, W.; Odaka, R.; Taguchi, H.; Ito, S.; Mori, H.; Matsui, H.
    Metabolic mechanism of mannan in a ruminal bacterium, Ruminococcus albus, involving two mannoside phosphorylases and cellobiose 2-epimerase: discovery of a new carbohydrate phosphorylase, beta-1,4-mannooligosaccharide phosphorylase (2012), J. Biol. Chem., 287, 42389-42399.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.281

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Ruminococcus albus

Inhibitors

Inhibitors Comment Organism Structure
alpha-D-mannose 1-phosphate competitive; competitive Ruminococcus albus
cellobiose
-
 Ruminococcus albus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.304
-
 4-O-beta-D-mannopyranosyl-D-glucopyranose pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
0.324
-
 alpha-D-mannose 1-phosphate pH 6.5, 37°C, synthetic reaction, cosubstrate D-glucose Ruminococcus albus
0.444
-
 phosphate pH 6.5, 37°C, phosphorolytic reaction, cosubstrate 4-O-beta-D-mannopyranosyl-D-glucopyranose Ruminococcus albus
5.34
-
 phosphate pH 6.5, 37°C, phosphorolytic reaction, cosubstrate 4-O-beta-D-mannopyranosyl-D-glucopyranose Ruminococcus albus
5.8
-
 alpha-D-mannose 1-phosphate pH 6.5, 37°C, synthetic reaction, cosubstrate D-glucose Ruminococcus albus
6.14
-
 D-allose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
6.44
-
 3-deoxy-3-fluoro-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
7.82
-
 3-O-methyl-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
22.2
-
 cellobiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
27.7
-
 4-O-beta-D-mannopyranosyl-D-glucopyranose pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
29.1
-
 laminaribiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
38.2
-
 D-glucose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
45.6
-
 D-mannose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
48.7
-
 2-deoxy-2-fluoro-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
49.8
-
 sophorose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
49.9
-
 N,N'-diacetylchitobiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
50.4
-
 maltose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
60.8
-
 gentiobiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
79.2
-
 1,5-anhydro-D-glucitol apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
85.2
-
 methyl beta-D-glucoside apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
85.3
-
 methyl alpha-D-glucoside apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
90.7
-
 2-deoxy-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
107
-
 N-acetyl-D-glucosamine apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
119
-
 6-deoxy-D-glucose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
156
-
 6-deoxy-6-fluoro-D-glucose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
172
-
 D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Ruminococcus albus 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37823
-
 6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence Ruminococcus albus
38000
-
 6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence Ruminococcus albus
43646
-
 2 * 45000, SDS-PAGE, 4 * 43646, amino acid sequence Ruminococcus albus
45000
-
 2 * 45000, SDS-PAGE, 4 * 43646, amino acid sequence Ruminococcus albus
80000
-
 gel filtration Ruminococcus albus
209000
-
 gel filtration Ruminococcus albus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate Ruminococcus albus
-
 D-glucose + alpha-D-mannose 1-phosphate
-
 r
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate Ruminococcus albus NE1
-
 D-glucose + alpha-D-mannose 1-phosphate
-
 r
6-deoxy-6-fluoro-D-glucose + alpha-D-mannose 1-phosphate Ruminococcus albus
-
 4-O-beta-D-mannopyranosyl-6-deoxy-6-fluoro-D-glucopyranose + phosphate
-
 ?
6-deoxy-6-fluoro-D-glucose + alpha-D-mannose 1-phosphate Ruminococcus albus NE1
-
 4-O-beta-D-mannopyranosyl-6-deoxy-6-fluoro-D-glucopyranose + phosphate
-
 ?
6-deoxy-D-glucose + alpha-D-mannose 1-phosphate Ruminococcus albus
-
 4-O-beta-D-mannopyranosyl-6-deoxy-D-glucopyranose + phosphate
-
 ?
6-deoxy-D-glucose + alpha-D-mannose 1-phosphate Ruminococcus albus NE1
-
 4-O-beta-D-mannopyranosyl-6-deoxy-D-glucopyranose + phosphate
-
 ?
D-glucose + alpha-D-mannose 1-phosphate Ruminococcus albus
-
 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
-
 r
D-glucose + alpha-D-mannose 1-phosphate Ruminococcus albus NE1
-
 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
-
 r
laminaribiose + alpha-D-mannose 1-phosphate Ruminococcus albus
-
 ? + phosphate
-
 ?
laminaribiose + alpha-D-mannose 1-phosphate Ruminococcus albus NE1
-
 ? + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Ruminococcus albus E6UBR9
-
-
Ruminococcus albus E6UIS7
-
-
Ruminococcus albus NE1 E6UBR9
-
-
Ruminococcus albus NE1 E6UIS7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ruminococcus albus

Reaction

Reaction Comment Organism Reaction ID
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate = D-glucose + alpha-D-mannose 1-phosphate sequential Bi Bi mechanism Ruminococcus albus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-anhydro-D-glucitol + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-1,5-anhydro-D-glucitol + phosphate
-
 r
1,5-anhydro-D-glucitol + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus NE1 4-O-beta-D-mannopyranosyl-1,5-anhydro-D-glucitol + phosphate
-
 r
2-deoxy-2-fluoro-D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-2-deoxy-2-fluoro-D-glucopyranose + phosphate
-
 r
2-deoxy-D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-2-deoxy-D-glucopyranose + phosphate
-
 r
3-deoxy-3-fluoro-D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-3-deoxy-3-fluoro-D-glucopyranose + phosphate
-
 r
3-O-methyl-D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-3-O-methyl-D-glucopyranose + phosphate
-
 r
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
-
 Ruminococcus albus D-glucose + alpha-D-mannose 1-phosphate
-
 r
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
-
 Ruminococcus albus NE1 D-glucose + alpha-D-mannose 1-phosphate
-
 r
6-deoxy-6-fluoro-D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-6-deoxy-6-fluoro-D-glucopyranose + phosphate
-
 ?
6-deoxy-6-fluoro-D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus NE1 4-O-beta-D-mannopyranosyl-6-deoxy-6-fluoro-D-glucopyranose + phosphate
-
 ?
6-deoxy-D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-6-deoxy-D-glucopyranose + phosphate
-
 ?
6-deoxy-D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus NE1 4-O-beta-D-mannopyranosyl-6-deoxy-D-glucopyranose + phosphate
-
 ?
cellobiose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus ? + phosphate
-
 r
D-allose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-D-allose + phosphate
-
 r
D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
-
 r
D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus NE1 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
-
 r
D-mannose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-D-mannopyranose + phosphate
-
 r
gentiobiose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus ? + phosphate
-
 r
laminaribiose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus ? + phosphate
-
 r
laminaribiose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus ? + phosphate
-
 ?
laminaribiose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus NE1 ? + phosphate
-
 r
laminaribiose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus NE1 ? + phosphate
-
 ?
maltose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus ? + phosphate
-
 r
methyl alpha-D-glucoside + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus methyl [4-O-beta-D-mannopyranosyl]-alpha-D-glucoside + phosphate
-
 r
methyl beta-D-glucoside + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus methyl [4-O-beta-D-mannopyranosyl]-beta-D-glucopyranoside + phosphate
-
 r
additional information The enzyme also catalyzes the reaction of beta-1,4-mannooligosaccharide phosphorylase Ruminococcus albus ?
-
 ?
additional information The enzyme also catalyzes the reaction of beta-1,4-mannooligosaccharide phosphorylase Ruminococcus albus NE1 ?
-
 ?
N,N'-diacetylchitobiose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus ? + phosphate
-
 r
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
-
 r
sophorose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus ? + phosphate
-
 r

Subunits

Subunits Comment Organism
homodimer 2 * 45000, SDS-PAGE, 4 * 43646, amino acid sequence Ruminococcus albus
homohexamer 6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence Ruminococcus albus

Synonyms

Synonyms Comment Organism
RaMp1
-
 Ruminococcus albus
RaMp2
-
 Ruminococcus albus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Ruminococcus albus
50
-
-
 Ruminococcus albus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable up to Ruminococcus albus
45
-
 stable up to Ruminococcus albus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.667
-
 D-allose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
0.763
-
 3-O-methyl-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
0.94
-
 3-deoxy-3-fluoro-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
5.07
-
 maltose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
5.12
-
 D-mannose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
5.71
-
 2-deoxy-2-fluoro-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
6.34
-
 N,N'-diacetylchitobiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
11.6
-
 laminaribiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
11.7
-
 2-deoxy-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
13.8
-
 sophorose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
15.8
-
 N-acetyl-D-glucosamine apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
17.5
-
 4-O-beta-D-mannopyranosyl-D-glucopyranose pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
20.2
-
 cellobiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
20.7
-
 1,5-anhydro-D-glucitol apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
20.7
-
 methyl alpha-D-glucoside apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
26.1
-
 gentiobiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
34.5
-
 D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
35.2
-
 methyl beta-D-glucoside apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
42
-
 6-deoxy-6-fluoro-D-glucose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
45.1
-
 D-glucose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
83.3
-
 4-O-beta-D-mannopyranosyl-D-glucopyranose pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
177
-
 6-deoxy-D-glucose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Ruminococcus albus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 9.5 4°C, 24 h stable Ruminococcus albus
4.5 10.5 4°C, 24 h stable Ruminococcus albus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0976
-
 3-O-methyl-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
0.101
-
 maltose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
0.109
-
 D-allose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
0.112
-
 D-mannose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
0.117
-
 2-deoxy-2-fluoro-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
0.127
-
 N,N'-diacetylchitobiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
0.129
-
 2-deoxy-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
0.146
-
 3-deoxy-3-fluoro-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
0.148
-
 N-acetyl-D-glucosamine apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
0.201
-
 D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
0.236
-
 methyl alpha-D-glucoside apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
0.261
-
 1,5-anhydro-D-glucitol apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
0.269
-
 6-deoxy-6-fluoro-D-glucose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
0.277
-
 sophorose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
0.399
-
 laminaribiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
0.413
-
 methyl beta-D-glucoside apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
0.429
-
 gentiobiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
0.91
-
 cellobiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
1.49
-
 6-deoxy-D-glucose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus