Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sulfhydryl reagents | required | Enterobacteria phage T6 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Enterobacteria phage T6 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + glucosylhydroxymethylcytosine residue in DNA | Enterobacteria phage T6 | - |
UDP + beta-D-glucosyl-beta-D-glucosyl-hydroxymethylcytosine containing DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacteria phage T6 | - |
Escherichia coli infected with | - |
Purification (Comment) | Organism |
---|---|
partial, using streptomycin sulfate treatment and fractionation on DEAE-cellulose | Enterobacteria phage T6 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0017 | - |
crude extract | Enterobacteria phage T6 |
0.0067 | - |
purified enzyme | Enterobacteria phage T6 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + glucosyl-5-hydroxymethylcytosine residue in DNA | no transfer of glucose to synthetic 5-hydroxymethylcytosine-DNA or to T6 DNA, enzyme glucosylates glucosylhydroxymethylcytosine residues of either alpha or beta configuration | Enterobacteria phage T6 | UDP + beta-D-glucosyl-beta-D-glucosyl-5-hydroxymethylcytosine containing DNA | - |
? | |
UDP-glucose + glucosylhydroxymethylcytosine residue in DNA | - |
Enterobacteria phage T6 | UDP + beta-D-glucosyl-beta-D-glucosyl-hydroxymethylcytosine containing DNA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Enterobacteria phage T6 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Enterobacteria phage T6 |