Literature summary for 2.4.1.278 extracted from

Yuan, Y.; Chung, H.S.; Leimkuhler, C.; Walsh, C.T.; Kahne, D.; Walker, S.
In vitro reconstitution of EryCIII activity for the preparation of unnatural macrolides (2005), J. Am. Chem. Soc., 127, 14128-14129.

Search for more literature for 2.4.1.278

Application

Application	Comment	Organism
medicine	the activated enzyme is capable of transferring an alternative unnatural sugar donor. Because the desosamine sugar contacts the 50S subunit of ribosome, the discovery that an unnatural sugar can be transferred has implications for the synthesis of new erythromycin derivatives with improved properties. Reconstitute of the activity of EryCIII in vitro for the synthesis of unnatural macrolides	Saccharopolyspora erythraea

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21(DE3) as a C-terminal His6 fusion protein	Saccharopolyspora erythraea

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	3-alpha-mycarosylerythronolide B	pH and temperature not specified in the publication, 0.05 mM dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose	Saccharopolyspora erythraea

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 3-alpha-mycarosylerythronolide B	Saccharopolyspora erythraea	dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose i.e. dTDP-D-desosamine	dTDP + erythromycin D	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharopolyspora erythraea	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharopolyspora erythraea

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 3-alpha-mycarosylerythronolide B	dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose i.e. dTDP-D-desosamine	Saccharopolyspora erythraea	dTDP + erythromycin D	-	?
dTDP-D-mycaminose + 3-alpha-mycarosylerythronolide B	the activated enzyme is capable of transferring an alternative sugar donor	Saccharopolyspora erythraea	dTDP + erythromycin M	-	?

Synonyms

Synonyms	Comment	Organism
EryCIII	-	Saccharopolyspora erythraea
glycosyltransferase EryCIII	-	Saccharopolyspora erythraea

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.017	-	3-alpha-mycarosylerythronolide B	pH and temperature not specified in the publication, 0.05 mM TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose	Saccharopolyspora erythraea

Cofactor

Cofactor	Comment	Organism	Structure
AKnT protein	the noncognate glycosyltransferase activator protein AknT, stably activates EryCIII. Addition of purified AknT to inactive EryCIII restores the activity of EryCIII in vitro	Saccharopolyspora erythraea
EryCII protein	glycosyltransferase activator protein EryCII activates EryCIII. EryCIII treated with EryCII is catalytically active, effecting transfer of desosamine to 3-alpha-mycarosylerythronolide. The untreated EryCIII sample shows no activity	Saccharopolyspora erythraea

General Information

General Information	Comment	Organism
physiological function	the enzyme is involved in erythromycin biosynthesis	Saccharopolyspora erythraea

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.34	-	3-alpha-mycarosylerythronolide B	pH and temperature not specified in the publication, 0.05 mM dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose	Saccharopolyspora erythraea

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Release 2023.1 (January 2023)