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Literature summary for 2.4.1.277 extracted from

  • Hong, J.S.; Park, S.J.; Parajuli, N.; Park, S.R.; Koh, H.S.; Jung, W.S.; Choi, C.Y.; Yoon, Y.J.
    Functional analysis of desVIII homologues involved in glycosylation of macrolide antibiotics by interspecies complementation (2006), Gene, 386, 123-130.
    View publication on PubMed
Search for more literature for 2.4.1.277

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 10-deoxymethynolide Streptomyces venezuelae
-
 dTDP + 10-deoxymethymycin
-
 ?
TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 10-deoxymethynolide Streptomyces venezuelae ATCC 15439
-
 dTDP + 10-deoxymethymycin
-
 ?
TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + narbonolide Streptomyces venezuelae
-
 dTDP + narbomycin
-
 ?
TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + narbonolide Streptomyces venezuelae ATCC 15439
-
 dTDP + narbomycin
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces venezuelae Q9ZGH7
-
-
Streptomyces venezuelae ATCC 15439 Q9ZGH7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 10-deoxymethynolide
-
 Streptomyces venezuelae dTDP + 10-deoxymethymycin
-
 ?
TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 10-deoxymethynolide
-
 Streptomyces venezuelae ATCC 15439 dTDP + 10-deoxymethymycin
-
 ?
TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + narbonolide
-
 Streptomyces venezuelae dTDP + narbomycin
-
 ?
TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + narbonolide
-
 Streptomyces venezuelae ATCC 15439 dTDP + narbomycin
-
 ?

Synonyms

Synonyms Comment Organism
macrolide glycosyltransferase DesVII
-
 Streptomyces venezuelae

Cofactor

Cofactor Comment Organism Structure
DesVIII protein DesVIII is an auxiliary protein which enhances the transfer of TDP-D-desosamine catalyzed by DesVII glycosyltransferase in the biosynthesis of macrolide antibiotics, neomethymycin, methymycin and pikromycin, in Streptomyces venezuelae ATCC 15439. The DesVIII homologues (except for TylMIII) can functionally replace the native DesVIII for glycosylation to proceed in vivo and their functions are similar in acting as glycosyltransferase auxiliary proteins. Complementation by expressing EryCII, OleP1, and DnrQ in a desVIII deletion mutant strain restors the production of glycosylated macrolides to an approximate level of 66%, 26% and 26%, respectively, compared to self-complementation by DesVIII Streptomyces venezuelae