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Literature summary for 2.4.1.277 extracted from

  • Borisova, S.A.; Zhang, C.; Takahashi, H.; Zhang, H.; Wong, A.W.; Thorson, J.S.; Liu, H.W.
    Substrate specificity of the macrolide-glycosylating enzyme pair DesVII/DesVIII: opportunities, limitations, and mechanistic hypotheses (2006), Angew. Chem. Int. Ed. Engl., 45, 2748-2753.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the recombinant DesVII protein is overproduced in Escherichia coli as a C-terminal His6-tagged fusion protein. The DesVIII protein is produced in Escherichia coli as a fusion with maltose-binding protein at its N-terminus. The fusion protein is cleaved by treatment with protease and untagged DesVIII protein is used in the assays together with DesVII Streptomyces venezuelae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 10-deoxymethynolide Streptomyces venezuelae
-
dTDP + 10-deoxymethymycin
-
?
TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + narbonolide Streptomyces venezuelae
-
dTDP + narbomycin
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces venezuelae Q9ZGH7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces venezuelae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 10-deoxymethynolide
-
Streptomyces venezuelae dTDP + 10-deoxymethymycin
-
?
TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 10-deoxymethynolide DesVII/DesVIII exhibits a stringent 6-deoxysugar requirement but an otherwise relaxed substrate specificity toward both TDP-sugar donor and aglycone acceptor Streptomyces venezuelae dTDP + 10-deoxymethymycin
-
?
TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + narbonolide
-
Streptomyces venezuelae dTDP + narbomycin
-
?
TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + narbonolide DesVII/DesVIII exhibits a stringent 6-deoxysugar requirement but an otherwise relaxed substrate specificity toward both TDP-sugar donor and aglycone acceptor Streptomyces venezuelae dTDP + narbomycin
-
?