Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Petrotoga mobilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | in the presence of 0.1, 2.0, or 5.0 mM EDTA, the enzyme retaines 85, 30, and 23% of its maximal activity, respectively | Petrotoga mobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | pH 8.5, 60°C | Petrotoga mobilis | |
1 | - |
GDP-mannose | pH 8.5, 60°C | Petrotoga mobilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | not strictly dependent on divalent cations, but the presence of 1 mM Mn2+, Ca2+, Mg2+, or Co2+ stimulates its activity in this order of efficiency | Petrotoga mobilis | |
Co2+ | not strictly dependent on divalent cations, but the presence of 1 mM Mn2+, Ca2+, Mg2+, or Co2+ stimulates its activity in this order of efficiency | Petrotoga mobilis | |
Mg2+ | not strictly dependent on divalent cations, but the presence of 1 mM Mn2+, Ca2+, Mg2+, or Co2+ stimulates its activity in this order of efficiency | Petrotoga mobilis | |
Mn2+ | not strictly dependent on divalent cations, but the presence of 1 mM Mn2+, Ca2+, Mg2+, or Co2+ stimulates its activity in this order of efficiency | Petrotoga mobilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
gel filtration | Petrotoga mobilis |
59000 | - |
1 * 59000, SDS-PAGE | Petrotoga mobilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | Petrotoga mobilis | the enzyme is involved in the phosphorylating pathway for synthesis of the solute mannosylglucosylglycerate. In Petrotoga mobilis two alternative pathways for the synthesis of the rare solute mannosylglucosylglycerate are proposed. The first one is a a phosphorylating pathway (with a phosphorylated intermediate) from 3-phosphoglycerate and UDP-glucose to the final solute. The second nonphosphorylating pathway (no phosphorylated intermediates) could represent an alternative route for the synthesis of mannosylglucosylglycerate in Petrotoga mobilis that could lead to the direct conversion of glucosylglycerate and GDP-mannose to mannosylglucosylglycerate. Pathway multiplicity likely reflects a crucial role for mannosylglucosylglycerate in the physiology of Petrotoga mobilis mobilis during stress adaptation | GDP + 2-(2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petrotoga mobilis | A9BHJ0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Petrotoga mobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | the enzyme is involved in the phosphorylating pathway for synthesis of the solute mannosylglucosylglycerate. In Petrotoga mobilis two alternative pathways for the synthesis of the rare solute mannosylglucosylglycerate are proposed. The first one is a a phosphorylating pathway (with a phosphorylated intermediate) from 3-phosphoglycerate and UDP-glucose to the final solute. The second nonphosphorylating pathway (no phosphorylated intermediates) could represent an alternative route for the synthesis of mannosylglucosylglycerate in Petrotoga mobilis that could lead to the direct conversion of glucosylglycerate and GDP-mannose to mannosylglucosylglycerate. Pathway multiplicity likely reflects a crucial role for mannosylglucosylglycerate in the physiology of Petrotoga mobilis mobilis during stress adaptation | Petrotoga mobilis | GDP + 2-(2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
GDP-mannose + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | no activity with UDP-mannose or mannose-1-phosphate as sugar donors, and 3-phospho-D-glycerate, 2-alpha-D-glucosylglycerate, and glucosyl-3-phosphoglycerate as the sugar acceptors | Petrotoga mobilis | GDP + 2-(2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 59000, SDS-PAGE | Petrotoga mobilis |
Synonyms | Comment | Organism |
---|---|---|
MggA | - |
Petrotoga mobilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Petrotoga mobilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 95 | 60°C: about 50% of maximal activity, 95°C: about 70% of maximal activity | Petrotoga mobilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life: 99 min | Petrotoga mobilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Petrotoga mobilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | pH 6.0: about 50% of maximal activity, pH 10.0: about 75% of maximal activity | Petrotoga mobilis |