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Literature summary for 2.4.1.268 extracted from

  • Fernandes, C.; Mendes, V.; Costa, J.; Empadinhas, N.; Jorge, C.; Lamosa, P.; Santos, H.; da Costa, M.S.
    Two alternative pathways for the synthesis of the rare compatible solute mannosylglucosylglycerate in Petrotoga mobilis (2010), J. Bacteriol., 192, 1624-1633.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Petrotoga mobilis

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, complete inhibition Petrotoga mobilis
Zn2+ strong inhibition Petrotoga mobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
D-glycerate pH 6.7, 60°C, native enzyme Petrotoga mobilis
1.9
-
D-glycerate pH 6.7, 70°C, native enzyme Petrotoga mobilis
2
-
ADP-glucose pH 6.7, 70°C, native enzyme Petrotoga mobilis
2.9
-
ADP-glucose pH 6.7, 60°C, native enzyme Petrotoga mobilis
3.4
-
D-glycerate pH 6.7, 60°C, recombinant enzyme Petrotoga mobilis
9.3
-
ADP-glucose pH 6.7, 60°C, recombinant enzyme Petrotoga mobilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 20 mM, stimulation. No stimulation by Mg2+, Ni2+, Co2+ Petrotoga mobilis
additional information NaCl and KCl have no effect on Ggs activity Petrotoga mobilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43700
-
4 * 43700, calculated from sequence Petrotoga mobilis
170000
-
gel filtration Petrotoga mobilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP-glucose + D-glycerate Petrotoga mobilis the enzyme is involved in the nonphosphorylating pathway for synthesis of the solute mannosylglucosylglycerate. In Petrotoga mobilis two alternative pathways for the synthesis of the solute mannosylglucosylglycerate are proposed. The first one is a phosphorylating pathway (with a phosphorylated intermediate) from 3-phosphoglycerate and UDP-glucose to the final solute. The second nonphosphorylating pathway (no phosphorylated intermediates) could represent an alternative route for the synthesis of mannosylglucosylglycerate in Petrotoga mobilis that could lead to the direct conversion of glucosylglycerate and GDP-mannose to mannosylglucosylglycerate. Pathway multiplicity likely reflects a crucial role for mannosylglucosylglycerate in the physiology of Petrotoga mobilis during stress adaptation 2-O-(alpha-D-glucopyranosyl)-D-glycerate + ADP
-
?

Organism

Organism UniProt Comment Textmining
Petrotoga mobilis A9BEU2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-glucose + D-glycerate the enzyme is involved in the nonphosphorylating pathway for synthesis of the solute mannosylglucosylglycerate. In Petrotoga mobilis two alternative pathways for the synthesis of the solute mannosylglucosylglycerate are proposed. The first one is a phosphorylating pathway (with a phosphorylated intermediate) from 3-phosphoglycerate and UDP-glucose to the final solute. The second nonphosphorylating pathway (no phosphorylated intermediates) could represent an alternative route for the synthesis of mannosylglucosylglycerate in Petrotoga mobilis that could lead to the direct conversion of glucosylglycerate and GDP-mannose to mannosylglucosylglycerate. Pathway multiplicity likely reflects a crucial role for mannosylglucosylglycerate in the physiology of Petrotoga mobilis during stress adaptation Petrotoga mobilis 2-O-(alpha-D-glucopyranosyl)-D-glycerate + ADP
-
?
ADP-glucose + D-glycerate formation of 2-O-(alpha-D-glucosyl)-D-glycerate is strongly favored. ADP-glucose is the preferred substrate for the recombinant glucosylglycerate synthase, which can be partially replaced by UDP-glucose with a very low efficiency. D-Glycerate is the only acceptor Petrotoga mobilis 2-O-(alpha-D-glucopyranosyl)-D-glycerate + ADP
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 43700, calculated from sequence Petrotoga mobilis

Synonyms

Synonyms Comment Organism
Ggs
-
Petrotoga mobilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70 75
-
Petrotoga mobilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
half-life: 3.1 h, recombinant enzyme Petrotoga mobilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
at 60°C Petrotoga mobilis