Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Petrotoga mobilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, complete inhibition | Petrotoga mobilis | |
Zn2+ | strong inhibition | Petrotoga mobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
D-glycerate | pH 6.7, 60°C, native enzyme | Petrotoga mobilis | |
1.9 | - |
D-glycerate | pH 6.7, 70°C, native enzyme | Petrotoga mobilis | |
2 | - |
ADP-glucose | pH 6.7, 70°C, native enzyme | Petrotoga mobilis | |
2.9 | - |
ADP-glucose | pH 6.7, 60°C, native enzyme | Petrotoga mobilis | |
3.4 | - |
D-glycerate | pH 6.7, 60°C, recombinant enzyme | Petrotoga mobilis | |
9.3 | - |
ADP-glucose | pH 6.7, 60°C, recombinant enzyme | Petrotoga mobilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 20 mM, stimulation. No stimulation by Mg2+, Ni2+, Co2+ | Petrotoga mobilis | |
additional information | NaCl and KCl have no effect on Ggs activity | Petrotoga mobilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43700 | - |
4 * 43700, calculated from sequence | Petrotoga mobilis |
170000 | - |
gel filtration | Petrotoga mobilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + D-glycerate | Petrotoga mobilis | the enzyme is involved in the nonphosphorylating pathway for synthesis of the solute mannosylglucosylglycerate. In Petrotoga mobilis two alternative pathways for the synthesis of the solute mannosylglucosylglycerate are proposed. The first one is a phosphorylating pathway (with a phosphorylated intermediate) from 3-phosphoglycerate and UDP-glucose to the final solute. The second nonphosphorylating pathway (no phosphorylated intermediates) could represent an alternative route for the synthesis of mannosylglucosylglycerate in Petrotoga mobilis that could lead to the direct conversion of glucosylglycerate and GDP-mannose to mannosylglucosylglycerate. Pathway multiplicity likely reflects a crucial role for mannosylglucosylglycerate in the physiology of Petrotoga mobilis during stress adaptation | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petrotoga mobilis | A9BEU2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + D-glycerate | the enzyme is involved in the nonphosphorylating pathway for synthesis of the solute mannosylglucosylglycerate. In Petrotoga mobilis two alternative pathways for the synthesis of the solute mannosylglucosylglycerate are proposed. The first one is a phosphorylating pathway (with a phosphorylated intermediate) from 3-phosphoglycerate and UDP-glucose to the final solute. The second nonphosphorylating pathway (no phosphorylated intermediates) could represent an alternative route for the synthesis of mannosylglucosylglycerate in Petrotoga mobilis that could lead to the direct conversion of glucosylglycerate and GDP-mannose to mannosylglucosylglycerate. Pathway multiplicity likely reflects a crucial role for mannosylglucosylglycerate in the physiology of Petrotoga mobilis during stress adaptation | Petrotoga mobilis | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + ADP | - |
? | |
ADP-glucose + D-glycerate | formation of 2-O-(alpha-D-glucosyl)-D-glycerate is strongly favored. ADP-glucose is the preferred substrate for the recombinant glucosylglycerate synthase, which can be partially replaced by UDP-glucose with a very low efficiency. D-Glycerate is the only acceptor | Petrotoga mobilis | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + ADP | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 43700, calculated from sequence | Petrotoga mobilis |
Synonyms | Comment | Organism |
---|---|---|
Ggs | - |
Petrotoga mobilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 75 | - |
Petrotoga mobilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life: 3.1 h, recombinant enzyme | Petrotoga mobilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
at 60°C | Petrotoga mobilis |