Cloned (Comment) | Organism |
---|---|
gene gpgS, DNA and amino acid sequence determination and analysis, phylogenetic tree | Mycobacterium tuberculosis |
gene gpgS, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in Escherichia coli strain BL21 | Mycobacterium tuberculosis variant bovis |
gene gpgS, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in Escherichia coli strain BL21 | Mycolicibacterium smegmatis |
overexpressed in Escherichia coli as a C-terminal His-tagged protein | Mycobacterium tuberculosis variant bovis |
overexpressed in Escherichia coli as a C-terminal His-tagged protein | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | GpgSs exhibits Michaelis-Menten kinetics at 37°C | Mycobacterium tuberculosis variant bovis | |
additional information | - |
additional information | GpgSs exhibits Michaelis-Menten kinetics at 37°C | Mycolicibacterium smegmatis | |
0.24 | - |
3-phospho-D-glycerate | pH 7.5, 37°C | Mycobacterium tuberculosis variant bovis | |
0.24 | - |
3-phospho-D-glycerate | pH 7.5, 37°C | Mycolicibacterium smegmatis | |
1.28 | - |
UDP-glucose | pH 7.5, 37°C | Mycolicibacterium smegmatis | |
2.43 | - |
UDP-glucose | pH 7.5, 37°C | Mycobacterium tuberculosis variant bovis | |
6.08 | - |
ADP-glucose | pH 7.5, 37°C | Mycolicibacterium smegmatis | |
6.63 | - |
ADP-glucose | pH 7.5, 37°C | Mycobacterium tuberculosis variant bovis | |
27.7 | - |
GDP-glucose | pH 7.5, 37°C | Mycobacterium tuberculosis variant bovis | |
32.04 | - |
GDP-glucose | pH 7.5, 37°C | Mycolicibacterium smegmatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | strictly dependent on | Mycobacterium tuberculosis | |
Mg2+ | strictly dependent on | Mycobacterium tuberculosis variant bovis | |
Mg2+ | strictly dependent on | Mycolicibacterium smegmatis | |
Mg2+ | activity is strictly dependent on Mg2+, maximal activation at 20 mM | Mycolicibacterium smegmatis | |
Mg2+ | activity is strictly dependent on Mg2+, maximal activation at 20-75 mM | Mycobacterium tuberculosis variant bovis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33900 | - |
3 * 33900, calculated from sequence | Mycolicibacterium smegmatis |
34400 | - |
3 * 34400, calculated from sequence | Mycobacterium tuberculosis variant bovis |
101100 | - |
gel filtration | Mycobacterium tuberculosis variant bovis |
106900 | - |
gel filtration | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 3-phospho-D-glycerate | Mycobacterium tuberculosis variant bovis | involved in biosynthesis of methylated polysaccharides | UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
UDP-glucose + 3-phospho-D-glycerate | Mycolicibacterium smegmatis | involved in biosynthesis of methylated polysaccharides | UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
UDP-glucose + 3-phospho-D-glycerate | Mycolicibacterium smegmatis ATCC 700084 | involved in biosynthesis of methylated polysaccharides | UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene gpgS or Rv1208 | - |
Mycobacterium tuberculosis H37Rv | - |
gene gpgS or Rv1208 | - |
Mycobacterium tuberculosis variant bovis | - |
- |
- |
Mycobacterium tuberculosis variant bovis | - |
slow-growing strain BCG, gene gpgS | - |
Mycolicibacterium smegmatis | A0R2E6 | fast-growing strain 1102, gene gpgS | - |
Mycolicibacterium smegmatis | A0R2E6 | strain 1102 | - |
Mycolicibacterium smegmatis ATCC 700084 | A0R2E6 | strain 1102 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Mycobacterium tuberculosis variant bovis |
recombinant enzyme from Escherichia coli | Mycolicibacterium smegmatis |
recombinant GpgS protein | Mycobacterium tuberculosis variant bovis |
recombinant GpgS protein | Mycolicibacterium smegmatis |
Storage Stability | Organism |
---|---|
0°C, purified recombinant enzyme, nearly 100% remaining activity after 1 month | Mycobacterium tuberculosis variant bovis |
0°C, purified recombinant enzyme, nearly 100% remaining activity after 1 month | Mycolicibacterium smegmatis |
22°C, room temperature, purified recombinant enzyme, nearly 100% remaining activity after 1 week | Mycobacterium tuberculosis variant bovis |
22°C, room temperature, purified recombinant enzyme, nearly 100% remaining activity after 1 week | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-alpha-D-glucose + D-3-phosphoglycerate | - |
Mycobacterium tuberculosis | ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
ADP-alpha-D-glucose + D-3-phosphoglycerate | - |
Mycobacterium tuberculosis variant bovis | ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
ADP-alpha-D-glucose + D-3-phosphoglycerate | - |
Mycolicibacterium smegmatis | ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
ADP-alpha-D-glucose + D-3-phosphoglycerate | - |
Mycolicibacterium smegmatis ATCC 700084 | ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
ADP-alpha-D-glucose + D-3-phosphoglycerate | - |
Mycobacterium tuberculosis H37Rv | ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
ADP-glucose + 3-phospho-D-glycerate | - |
Mycobacterium tuberculosis variant bovis | ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
ADP-glucose + 3-phospho-D-glycerate | - |
Mycolicibacterium smegmatis | ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
GDP-glucose + 3-phospho-D-glycerate | - |
Mycobacterium tuberculosis variant bovis | GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
GDP-glucose + 3-phospho-D-glycerate | - |
Mycolicibacterium smegmatis | GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
GDP-glucose + 3-phospho-D-glycerate | - |
Mycolicibacterium smegmatis ATCC 700084 | GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
UDP-glucose + 3-phospho-D-glycerate | - |
Mycobacterium tuberculosis variant bovis | UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
UDP-glucose + 3-phospho-D-glycerate | - |
Mycolicibacterium smegmatis | UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
UDP-glucose + 3-phospho-D-glycerate | involved in biosynthesis of methylated polysaccharides | Mycobacterium tuberculosis variant bovis | UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
UDP-glucose + 3-phospho-D-glycerate | involved in biosynthesis of methylated polysaccharides | Mycolicibacterium smegmatis | UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
UDP-glucose + 3-phospho-D-glycerate | - |
Mycolicibacterium smegmatis ATCC 700084 | UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
UDP-glucose + 3-phospho-D-glycerate | involved in biosynthesis of methylated polysaccharides | Mycolicibacterium smegmatis ATCC 700084 | UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
UDP-glucose + D-3-phosphoglycerate | best substrates | Mycobacterium tuberculosis | UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate | - |
? | |
UDP-glucose + D-3-phosphoglycerate | best substrates | Mycobacterium tuberculosis variant bovis | UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate | - |
? | |
UDP-glucose + D-3-phosphoglycerate | best substrates | Mycolicibacterium smegmatis | UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate | - |
? | |
UDP-glucose + D-3-phosphoglycerate | best substrates | Mycolicibacterium smegmatis ATCC 700084 | UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate | - |
? | |
UDP-glucose + D-3-phosphoglycerate | best substrates | Mycobacterium tuberculosis H37Rv | UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 33900, calculated from sequence | Mycolicibacterium smegmatis |
trimer | 3 * 34400, calculated from sequence | Mycobacterium tuberculosis variant bovis |
Synonyms | Comment | Organism |
---|---|---|
glucosyl-3-phosphoglycerate synthase | - |
Mycobacterium tuberculosis |
glucosyl-3-phosphoglycerate synthase | - |
Mycobacterium tuberculosis variant bovis |
glucosyl-3-phosphoglycerate synthase | - |
Mycolicibacterium smegmatis |
GpgS | - |
Mycobacterium tuberculosis |
GpgS | - |
Mycobacterium tuberculosis variant bovis |
GpgS | - |
Mycolicibacterium smegmatis |
GpgS protein | - |
Mycobacterium tuberculosis variant bovis |
GpgS protein | - |
Mycolicibacterium smegmatis |
More | the enzyme belongs to the retaining family 81 glycosyltransferases | Mycobacterium tuberculosis |
More | the enzyme belongs to the retaining family 81 glycosyltransferases | Mycobacterium tuberculosis variant bovis |
More | the enzyme belongs to the retaining family 81 glycosyltransferases | Mycolicibacterium smegmatis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Mycobacterium tuberculosis |
45 | - |
- |
Mycobacterium tuberculosis variant bovis |
45 | - |
- |
Mycolicibacterium smegmatis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 55 | no activity below 20°C and above 55°C | Mycobacterium tuberculosis |
20 | 55 | no activity below 20°C and above 55°C | Mycobacterium tuberculosis variant bovis |
20 | 55 | no activity below 20°C and above 55°C | Mycolicibacterium smegmatis |
30 | 50 | 30°C: about 40% of maximal activity, 50°C: about 70% of maximal activity | Mycolicibacterium smegmatis |
35 | 50 | 30°C: about 35% of maximal activity, 35°C: about 60% of maximal activity, 50°C: about 90% of maximal activity, 55°C: about 25% of maximal activity | Mycobacterium tuberculosis variant bovis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
half-life value for inactivation of GpgS is 12.42 h | Mycobacterium tuberculosis variant bovis |
37 | - |
half-life value for inactivation of GpgS is 7.28 h | Mycolicibacterium smegmatis |
37 | - |
half-life 7.28 h | Mycolicibacterium smegmatis |
37 | - |
half-life: 12.42 h | Mycobacterium tuberculosis variant bovis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Mycobacterium tuberculosis |
8 | - |
- |
Mycobacterium tuberculosis variant bovis |
8 | - |
- |
Mycolicibacterium smegmatis |
8.5 | - |
- |
Mycolicibacterium smegmatis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 50% of maximal activity, pH 9.5: about 75% of maximal activity | Mycobacterium tuberculosis variant bovis |
6 | 9.5 | pH 6.0: about 55% of maximal activity, pH 9.5: about 60% of maximal activity | Mycolicibacterium smegmatis |
6 | 10 | activity range | Mycobacterium tuberculosis variant bovis |
6 | 10 | activity range | Mycolicibacterium smegmatis |