General Stability | Organism |
---|---|
CHAPS > deoxy-BIGCHAP > n-octyl-beta-D-thioglucoside. Sodium cholate, SM-1200, and n-dodecyl-beta-D-maltoside do not effectively solubilize UGASGT | Glycine max |
the effectiveness of detergents at the CMC in solubilizing UGASGT is as follows: MEGA10 > SM-1000 > | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mega-10 | the detergent inactivates UGASGT during solubilization | Glycine max | |
SM-1000 | the detergent inactivates UGASGT during solubilization | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00403 | - |
soyasapogenol A | in 100 mM Tris-HCl (pH 8.0), at 40°C | Glycine max | |
0.01722 | - |
soyasapogenol B | in 100 mM Tris-HCl (pH 8.0), at 40°C | Glycine max | |
0.02394 | - |
UDP-D-glucuronate | using soyasapogenol B as the sugar acceptor, in 100 mM Tris-HCl (pH 8.0), at 40°C | Glycine max | |
0.04194 | - |
soyasapogenol E | in 100 mM Tris-HCl (pH 8.0), at 40°C | Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Glycine max | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the effectiveness of divalent cations at 10 mM in activating UGASGT is as follows: Mg2+>Mn2+>Ca2+ | Glycine max | |
Mg2+ | the optimum concentration of Mg2+ is between 10 and 25 mM. UGASGT shows an absolute requirement for divalent cations. The effectiveness of divalent cations at 10 mM in activating UGASGT is as follows: Mg2+>Mn2+>Ca2+ | Glycine max | |
Mn2+ | the effectiveness of divalent cations at 10 mM in activating UGASGT is as follows: Mg2+>Mn2+>Ca2+ | Glycine max | |
additional information | Na+, K+, Co2+, Fe2+, Zn2+, Cu2+, Al3+, and Fe3+, activate UGASGT only slightly or not at all | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
the 66000 Da and/or the 50000 Da proteins are the UGASGT protein as determined by SDS-PAGE | Glycine max |
66000 | - |
the 66000 Da and/or the 50000 Da proteins are the UGASGT protein as determined by SDS-PAGE | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Q column chromatography, Superdex 200 gel filtration, and HiTrap Blue column chromatography column chromatography | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.342 | - |
crude extract, in 100 mM Tris-HCl (pH 8.0), at 40°C | Glycine max |
7 | - |
after 204.68fold purification, in 100 mM Tris-HCl (pH 8.0), at 40°C | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | beta-amyrin, sophoradiol, and glycyrrhetinic acid are not converted by UGASGT. UGASGT does not catalyze the transfer reaction of glucuronate to soyasapogenol B-3-O-beta-glucuronide | Glycine max | ? | - |
? | |
UDP-D-glucuronate + soyasapogenol A | 40.6% activity compared to soyasapogenol B | Glycine max | UDP + soyasapogenol A 3-O-D-glucuronide | - |
? | |
UDP-D-glucuronate + soyasapogenol B | soyasapogenol B is the best sugar acceptor, 100% activity | Glycine max | UDP + soyasapogenol B 3-O-D-glucuronide | - |
? | |
UDP-D-glucuronate + soyasapogenol E | 79.08% activity compared to soyasapogenol B | Glycine max | UDP + soyasapogenol E 3-O-D-glucuronide | - |
? | |
UDP-galactose + soyasapogenol B | 8.35% activity compared to UDP-D-glucuronate | Glycine max | ? | - |
? | |
UDP-glucose + soyasapogenol B | 7.94% activity compared to UDP-D_glucuronate | Glycine max | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucuronic acid:soyasapogenol glucuronosyltransferase | - |
Glycine max |
UGASGT | - |
Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
in Tris-HCl buffer (pH 8.0) | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Glycine max |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Glycine max |