Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.252 extracted from

  • Geremia, R.A.; Roux, M.; Ferreiro, D.U.; Dauphin-Dubois, R.; Lellouch, A.C.; Ielpi, L.
    Expression and biochemical characterisation of recombinant AceA, a bacterial alpha-mannosyltransferase (1990), Mol. Gen. Genet., 261, 933-940.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overproduced in Escherichia coli. The aceA ORF is subcloned into the expression vector pET29 in frame with the S-tag epitope. The recombinant protein is identified, and culture conditions are optimised for production of the soluble protein Komagataeibacter xylinus

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by cellobiose Komagataeibacter xylinus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol Komagataeibacter xylinus the enzyme is involved in the biosynthesis of the exopolysaccharide acetan GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
-
?

Organism

Organism UniProt Comment Textmining
Komagataeibacter xylinus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol the enzyme is involved in the biosynthesis of the exopolysaccharide acetan Komagataeibacter xylinus GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
-
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol AceA is not able to use free cellobiose as a substrate, indicating that the pyrophosphate-lipid moiety is needed for enzymatic activity Komagataeibacter xylinus GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol characterisation of the in vitro reaction product ?

Synonyms

Synonyms Comment Organism
AceA
-
Komagataeibacter xylinus

General Information

General Information Comment Organism
physiological function the enzyme is involved in the biosynthesis of the exopolysaccharide acetan Komagataeibacter xylinus