Cloned (Comment) | Organism |
---|---|
overproduced in Escherichia coli. The aceA ORF is subcloned into the expression vector pET29 in frame with the S-tag epitope. The recombinant protein is identified, and culture conditions are optimised for production of the soluble protein | Komagataeibacter xylinus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by cellobiose | Komagataeibacter xylinus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol | Komagataeibacter xylinus | the enzyme is involved in the biosynthesis of the exopolysaccharide acetan | GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataeibacter xylinus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol | the enzyme is involved in the biosynthesis of the exopolysaccharide acetan | Komagataeibacter xylinus | GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol | - |
? | |
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol | AceA is not able to use free cellobiose as a substrate, indicating that the pyrophosphate-lipid moiety is needed for enzymatic activity | Komagataeibacter xylinus | GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol | characterisation of the in vitro reaction product | ? |
Synonyms | Comment | Organism |
---|---|---|
AceA | - |
Komagataeibacter xylinus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of the exopolysaccharide acetan | Komagataeibacter xylinus |