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Literature summary for 2.4.1.252 extracted from

  • Katzen, F.; Ferreiro, D.U.; Oddo, C.G.; Ielmini, M.V.; Becker, A.; Pühler, A.; Ielpi, L.
    Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence (1998), J. Bacteriol., 180, 1607-1617.
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
synthesis the enzyme is involved in biosynthesis of xanthan, an industrially important exopolysaccharide Xanthomonas campestris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol Xanthomonas campestris the enzyme is involved in biosynthesis of xanthan GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol
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Organism

Organism UniProt Comment Textmining
Xanthomonas campestris
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 pv. campestris
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol
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 Xanthomonas campestris GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol
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 ?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol the enzyme is involved in biosynthesis of xanthan Xanthomonas campestris GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol
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 ?

Synonyms

Synonyms Comment Organism
gumH
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 Xanthomonas campestris

General Information

General Information Comment Organism
malfunction from the biochemical analysis of a defined set of Xanthomonas campestris gum mutants, experimental data are reported for assigning functions to the products of the gum genes. Inactivation of gumH completely abolishes in vitro polymer formation. Permeabilized cells are incubated with UDP-glucose, GDPmannose, and UDP-glucuronic acid, one of them labeled in the sugar moiety. Accumulating intermediates are analysed. Oligosaccharides obtained from gumH- strain XcH can be resolved into two components: a compound with the same mobility as the disaccharide cellobiose and glucose. XcH cells labeled with UDP-[14C]glucuronic acid or GDP-[14C]mannose do not incorporate radioactivity into the 1203 extract Xanthomonas campestris
physiological function the enzyme is involved in biosynthesis of xanthan Xanthomonas campestris