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Literature summary for 2.4.1.25 extracted from
- Gene cloning, expression enhancement in Escherichia coli and biochemical characterization of a highly thermostable amylomaltase from Pyrobaculum calidifontis (2020), Int. J. Biol. Macromol., 165, 645-653.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | recombinant Pcal_0768 is successfully employed in the synthesis of modified starch for producing thermo-reversible gel | Pyrobaculum calidifontis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Pcal_0768, DNA and amino acid sequence determination and analysis, recombinant expression of soluble and active enzyme in Escherichia coli | Pyrobaculum calidifontis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the expression level of the recombinant enzyme in Escherichia coli is not very high. Analysis of the mRNA of initial seven codons at the 5'-end of the gene reveals the presence of a hair pin like secondary structure. This secondary structure is removed by site-directed mutagenesis, without altering the amino acids, which results in enhanced expression of the cloned gene | Pyrobaculum calidifontis |
General Stability
| General Stability | Organism |
|---|---|
| no metal ions are required for stability of the enzyme | Pyrobaculum calidifontis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no metal ions are required for activity or stability of the enzyme | Pyrobaculum calidifontis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrobaculum calidifontis | A3MU77 | - |
- |
| Pyrobaculum calidifontis DSM 21063 | A3MU77 | - |
- |
| Pyrobaculum calidifontis JCM 11548 | A3MU77 | - |
- |
| Pyrobaculum calidifontis VA1 | A3MU77 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 690 | - |
purified recombinant enzyme, pH 6.9, 80°C | Pyrobaculum calidifontis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| amylomaltase | - |
Pyrobaculum calidifontis |
| Pcal_0768 | - |
Pyrobaculum calidifontis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
recombinant enzyme | Pyrobaculum calidifontis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
purified recombinant enzyme, half-life is 6 h | Pyrobaculum calidifontis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
recombinant enzyme | Pyrobaculum calidifontis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycoside hydrolase family 77, GH77 | Pyrobaculum calidifontis |
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