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Literature summary for 2.4.1.25 extracted from

  • Kaulpiboon, J.; Rudeekulthamrong, P.
    Maltotriosyl-erythritol, a transglycosylation product of erythritol by Thermus sp. amylomaltase and its application to prebiotic (2025), Food Chem., 472, 142937.
    View publication on PubMed
Search for more literature for 2.4.1.25

Application

Application Comment Organism
synthesis erythritol-receptor products formed via AMase-catalyzed reactions, particularly maltotriosyl-erythritol (EG3), are promising prebiotic ingredients, with the prebiotic activity of erythritol derivatives being influenced by the length of the carbohydrate chain. Fermentation of EG3 results in the highest levels of lactic acid and total short-chain fatty acids, which may contribute to reduced pH levels Thermus sp.

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Thermus sp.

Organism

Organism UniProt Comment Textmining
Thermus sp.
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-
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Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Thermus sp.

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Thermus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
120
-
 purified recombinant enzyme, starch transglycosylation assay, pH 6.0, 60°C Thermus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information scale-up preparation and purification of synthesized maltotriosyl-erythritol (EG3) derivatives, overview. The main transfer product of 1-O- and 4-O-forms may be a mixture of equal amounts, EG3 ought to be connected to erythritol through three alpha-configurations as a result of the particular transglycosylation activity of alpha-AMase. Generally, it seems that the type of alpha- or beta-glycosidic linkage that formed between the acceptor and donor is based on the enzyme that is used in the transglycosylation reaction Thermus sp. ?
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starch + erythritol maltotriosyl-erythritol (EG3) is synthesized via transglycosylation by recombinant amylomaltase (AMase), tapioca starch serves as the glucosyl donor, and erythritol as the acceptor. HPLC analysis reveals an EG3 yield of 14.0% with a concentration of 2.8 mg/ml. Mass spectrometry confirms the molecular weight of EG3 as 608 Da, and its structure is verified by 1H and 13C NMR analysis. Starch transglycosylation assay Thermus sp. maltotriosyl-erythritol + ?
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 ?

Synonyms

Synonyms Comment Organism
alpha-AMase
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 Thermus sp.
AMase
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 Thermus sp.
amylomaltase
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 Thermus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Thermus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Thermus sp.